{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T16:54:14Z","timestamp":1783961654377,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,10,27]],"date-time":"2024-10-27T00:00:00Z","timestamp":1729987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101047"],"award-info":[{"award-number":["82101047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>VECs play a crucial role in regulating the function of neutrophils, which is essential for immune responses and inflammation. As stretch\u2010sensitive cells, VECs sense mechanical stretch through surface mechanoreceptors, converting external mechanical stimuli into biochemical signals. This study aimed to explore the molecular mechanisms underlying the regulation of neutrophil behavior by stretched VECs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The key cytokine\u2010inducing neutrophil N2 polarization in the conditioned medium from stretched vascular endothelial cells (CM\u2010stretch) was validated through multifactorial matrix and flow cytometry. Additionally, the molecular mechanism underlying the response of vascular endothelial cells to stretch was systematically verified through layer\u2010by\u2010layer analysis using WB.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IL13, not IL4, was ultimately identified as a key cytokine\u2010inducing neutrophil N2 polarization in CM\u2010stretch. Inhibition of the transient receptor potential channel (TRPC1) and siRNA\u2010mediated knockdown of TRPC1 both significantly decreased IL13 production. Furthermore, neutralizing IL13 in the CM\u2010stretch or inhibiting STAT3 phosphorylation inhibited neutrophil N2 polarization, as evidenced by reduced CD206 and VEGFA expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results demonstrate that stretched VECs initiate a signaling cascade that induces neutrophil N2 polarization through the TRPC1\u2010IL13\u2010STAT3 axis, suggesting that mechanical stretching of VECs could shift neutrophil function from a pro\u2010inflammatory to a more regulatory and healing role.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.15168","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T03:48:28Z","timestamp":1730087308000},"page":"918-927","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Stretched Vascular Endothelial Cells Polarized Neutrophils to <scp>N2<\/scp> Type via <scp>TRPC1<\/scp>\u2010<scp>IL13<\/scp>\u2010<scp>STAT3<\/scp> Axis"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2276-9174","authenticated-orcid":false,"given":"Ting","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine Chinese Academy of Medical Sciences  Shanghai China"},{"name":"Shanghai Engineering Research Center of Advanced Dental Technology and Materials National Clinical Research Center of Stomatology  Shanghai 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Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine Chinese Academy of Medical Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohua","family":"Tang","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Advanced Dental Technology and Materials National Clinical Research Center of Stomatology  Shanghai China"},{"name":"Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine Chinese Academy of Medical Sciences  Shanghai 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