{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:53:57Z","timestamp":1772160837947,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T00:00:00Z","timestamp":1730160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The complement cascade plays an important role in the inflammation amplification and tissue destruction of periodontitis. Importantly, complement C3 was proved to be the central element of complement cascade. Thus, targeting inhibition of C3 has become one of the focuses of treatment method development and exploration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The siRNAs targeting C3 were designed and screened for in\u00a0vitro potency. The selected siRNA was conjugated to GalNAc (GalNAc\u2010C3 siRNA) for liver\u2010specific delivery. The mouse model of periodontitis was established by silk ligation. Stereomicroscopy, Micro\u2010CT, histological and histochemical assessment, and immunofluorescence staining were performed to evaluate the level of bone destructive and osteoclast activity. The influence of GalNAc\u2010C3 siRNA on inflammatory reactions was determined by qRT\u2010PCR, ELISA, and flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>GalNAc\u2010C3 siRNA showed great in\u00a0vivo potency and durability to silence hepatic C3 mRNA expression. GalNAc\u2010C3 siRNA treatment could effectively inhibit the production of inflammatory cytokines (IL\u201017A, TNF\u2010\u03b1, IL\u20106, and IFN\u2010\u03b3) and restrain Th17 differentiation. Importantly, the expression of RANKL and differentiation of osteoclast were inhibited by GalNAc\u2010C3 siRNA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>GalNAc\u2010C3 siRNA could efficiently play a role in bone protection by inhibiting inflammatory responses and osteoclast activities. This therapeutic siRNA may become an effective treatment strategy for periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.15170","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:50:14Z","timestamp":1730249414000},"page":"589-599","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>GalNAc<\/scp>\n                    \u2010Conjugated\n                    <scp>siRNA<\/scp>\n                    Targeting Complement\n                    <scp>C3<\/scp>\n                    Inhibits Osteoclast Activation in Periodontitis"],"prefix":"10.1111","volume":"31","author":[{"given":"Yingyi","family":"Chen","sequence":"first","affiliation":[{"name":"Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology Capital Medical University  Beijing People's Republic of China"},{"name":"Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yitong","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology Capital Medical University  Beijing People's Republic of China"},{"name":"Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongguo","family":"Fu","sequence":"additional","affiliation":[{"name":"Suzhou Ribo Life Science co. Ltd.  Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junji","family":"Xu","sequence":"additional","affiliation":[{"name":"Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology Capital Medical University  Beijing People's Republic of China"},{"name":"Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijia","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, School of Stomatology Capital Medical University  Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiqing","family":"Cao","sequence":"additional","affiliation":[{"name":"Suzhou Ribo Life Science co. Ltd.  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