{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:10:05Z","timestamp":1780449005326,"version":"3.54.1"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T00:00:00Z","timestamp":1735603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001066"],"award-info":[{"award-number":["82001066"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>To meet their high energy needs, tumor cells undergo aberrant metabolic reprogramming. A tumor cell may expertly modify its metabolic pathways and the differential expression of the genes for metabolic enzymes. The physiological requirements of the host tissue and the tumor cell of origin mostly dictate metabolic adaptation. Ameloblastoma (AB) is a benign odontogenic tumor of epithelial origin. Due to its unrestricted growth potential, local aggressiveness, and high likelihood of recurrence, this condition poses a significant risk to the patient's health. This study aimed to characterize the metabolic heterogeneity at single\u2010cell resolution of AB.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Single\u2010cell RNA sequencing (scRNA\u2010seq) was performed on 17,284 cells from three AB donors. Bioinformatic analysis was used to examine differentially expressed genes, subtypes, and regulatory mechanisms when combined with odontogenic keratocyst scRNA\u2010seq data. Based on metabolic pathway gene sets, the metabolic landscape of AB tumor cells was examined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Using scRNA\u2010seq, we discovered that AB tumor cells had substantial heterogeneity. The biggest contributor to tumor cell metabolic characteristics is determined to be variation in mitochondrial programming and glycolysis. Surprisingly, hypoxia corresponds with both oxidative phosphorylation and glycolysis activity in AB tumor cells at the single\u2010cell level.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study presents a computational framework for defining metabolism using single\u2010cell expression data and identifies oxidative phosphorylation and glycolysis as critical components of metabolism for AB tumor cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.15239","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T07:16:06Z","timestamp":1735629366000},"page":"2206-2214","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Metabolic Analysis of Tumor Cells Within Ameloblastoma at the Single\u2010Cell Level"],"prefix":"10.1111","volume":"31","author":[{"given":"Rui\u2010Fang","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8478-2086","authenticated-orcid":false,"given":"Qi\u2010Wen","family":"Man","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Oral and Maxillofacial Head Neck Oncology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,31]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jobcr.2020.07.008"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4314\/ahs.v14i1.9"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15965"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3124"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.08.016"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)84944-4"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2022.01.605"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-017-2090-6"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2016.01.007"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab012"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1173"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12646"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cancerbio-050216-121954"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.69"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2016.6750"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2013.02.003"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.694"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2017.0096"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP280572"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0619-0"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.4132\/KoreanJPathol.2013.47.3.191"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2015.12.001"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2017.09.001"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.16110"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1590\/2177-6709.24.4.021-032.oin"},{"issue":"1","key":"e_1_2_10_29_1","first-page":"S167","article-title":"Ameloblastoma","volume":"7","author":"Masthan K. 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