{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:54:09Z","timestamp":1772160849839,"version":"3.50.1"},"reference-count":78,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Primary Sj\u00f6gren's disease (SjD) is a slow\u2010progressing autoimmune disease that affects salivary and lacrimal glands, causing dry eyes (xerophthalmia) and dry mouth (xerostomia). Diagnosing SjD involves clinical, serological, and histological assessments, but reliable biomarkers are lacking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study analyzed the metabolic and proteomic profiles of 19 female SjD patients (based on ACR\u2010EULAR criteria) compared to 20 healthy individuals. Saliva and blood samples were analyzed using mass spectrometry and chromatography. Proteomic analysis was performed with the nanoElute nanoflow system coupled to a timsTof\u2010Pro mass spectrometer, and metabolic profiling with a GC\u2010TOF\/MS Pegasus HT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Statistical tests identified significant differences in metabolites and proteins between SjD patients and controls. Metabolome analysis revealed changes in amino acid synthesis, purine and lipid metabolism, and exposure to external compounds. Proteomic analysis indicated immune\u2010related proteins and inflammatory lipid metabolism. GNAI2, B2MG, NGAL, SLUR2, HS90, SODC, and A2GL emerged as potential biomarkers for SjD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study demonstrates the potential of high\u2010performance techniques in identifying biomarkers for SjD diagnosis and prognosis. Data are available via ProteomeXchange (PXD055629).<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.15328","type":"journal-article","created":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T00:47:15Z","timestamp":1743295635000},"page":"2474-2486","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Genomics Using High\u2010Throughput Methods for Salivary Analysis in Sj\u00f6gren's Disease"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5328-0937","authenticated-orcid":false,"given":"Giovanna P.","family":"Florezi","sequence":"first","affiliation":[{"name":"Stomatology Department, Faculdade de Odontologia Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6447-3718","authenticated-orcid":false,"given":"M\u00f4nica Teresa Veneziano","family":"Labate","sequence":"additional","affiliation":[{"name":"Genetics Department \u2013 Escola Superior de Agricultura Luiz de Queiroz Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7309-1300","authenticated-orcid":false,"given":"Carlos Alberto","family":"Labate","sequence":"additional","affiliation":[{"name":"Genetics Department \u2013 Escola Superior de Agricultura Luiz de Queiroz Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5394-7623","authenticated-orcid":false,"given":"Felippe P.","family":"Barone","sequence":"additional","affiliation":[{"name":"Stomatology Department, Faculdade de Odontologia Universidade de S\u00e3o Paulo  S\u00e3o Paulo 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