{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:54:03Z","timestamp":1772160843605,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T00:00:00Z","timestamp":1745193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to investigate the specific role and mechanistic actions of tumor necrosis factor receptor\u2010associated factor 4 (TRAF4) in periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Human periodontal ligament stem cells (PDLSCs) were exposed to lipopolysaccharide (LPS). Then, real\u2010time quantitative polymerase chain reaction (RT\u2010qPCR) and western blotting (WB) were carried out to determine the mRNA and protein expression levels of Smad ubiquitination regulator 1 (SMURF1). The relationship between TRAF4 and SMURF1, as predicted by the STRING and GeneMANIA databases, was verified by co\u2010immunoprecipitation (Co\u2010IP). Finally, both TRAF4 and SMURF1 were inhibited in PDLSCs by cell transfection, and the regulatory mechanisms involved were investigated by cell counting kit\u20108 assays, enzyme linked immunosorbent assay, WB, alkaline phosphatase, and alizarin red staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The gene and protein expression levels of SMURF1 in PDLSCs increased following LPS induction (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001); cell viability was decreased (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), TRAF4 expression was decreased (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), and cell\u2010mineralized nodules were inhibited. Inhibition of SMURF1 expression increased PDLSCs activity and TRAF4 expression levels (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), increased the number of cell\u2010mineralized nodules, and enhanced cellular osteogenic capacity (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>SMURF1 regulates LPS\u2010stimulated injury and improves the capacity for osteogenic differentiation in PDLSCs by downregulating the expression of TRAF4.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.15341","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T02:00:15Z","timestamp":1745287215000},"page":"2572-2583","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>SMURF1<\/scp>\n                    Regulates Periodontal Stem Cell Injury and Osteogenic Differentiation by Regulating\n                    <scp>TRAF4<\/scp>"],"prefix":"10.1111","volume":"31","author":[{"given":"Ziming","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Orthodontics, Stomatological Hospital, School of Stomatology Southern Medical University  Guangzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Stomatological Hospital, School of Stomatology Southern Medical University  Guangzhou People's Republic of 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