{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:41:12Z","timestamp":1790023272215,"version":"4.0.1"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010724","name":"Health Systems Research Institute","doi-asserted-by":"publisher","award":["68\u2010032,68\u2010059"],"award-info":[{"award-number":["68\u2010032,68\u2010059"]}],"id":[{"id":"10.13039\/501100010724","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"publisher","award":["HEA_FF_68_008_3200_001,HEA_FF_68_223_3200_015"],"award-info":[{"award-number":["HEA_FF_68_008_3200_001,HEA_FF_68_223_3200_015"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100023915","name":"Faculty of Dentistry, Chulalongkorn University","doi-asserted-by":"publisher","award":["DRF68_007"],"award-info":[{"award-number":["DRF68_007"]}],"id":[{"id":"10.13039\/501100023915","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Oral lichen planus (OLP) is a chronic inflammatory condition often associated with desquamative gingivitis (DG). The oral microbiome's role in OLP and DG (OLP\u2010DG) is gaining recognition, but prior 16S rRNA studies lacked taxonomic resolution. This study introduced shotgun metagenomic sequencing to thoroughly compare the supragingival and subgingival plaque microbiomes of individuals with and without OLP\u2010DG.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Twenty\u2010seven participants (9 OLP\u2010DG, 18 non\u2010OLP) were recruited. Supra\u2010 and subgingival plaque samples were collected separately. Genomic DNA was analyzed using shotgun metagenomic sequencing. Microbial abundance and diversity were assessed through bioinformatic and statistical analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We observed significant changes in the supragingival and subgingival microbiomes in OLP\u2010DG. Supragingival plaque showed reduced Corynebacteriaceae and Porphyromonadaceae, with enrichment of an unnamed Synergistaceae genus and three unnamed species (Candidatus Saccharibacteria bacterium oral taxon 955 and 488 and GGB10852_SGB17523). Subgingival plaque revealed increased Flavobacteriaceae and Rhodocyclaceae, and reduced Actinomycetaceae. Although alpha or beta diversity was not significantly different, common commensals like\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Corynebacterium matruchotii<\/jats:italic>\n                      <\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Streptococcus mitis<\/jats:italic>\n                      <\/jats:styled-content>\n                      were less abundant in OLP\u2010DG patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This first\u2010time application of metagenomic sequencing revealed a distinct microbiome in OLP\u2010DG, characterized by novel bacterial species and reduced commensals, suggesting a potential role in OLP\u2010DG pathogenesis, and warranting further study.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.15349","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T01:15:19Z","timestamp":1745543719000},"page":"3069-3080","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Shotgun Metagenomics of Biofilm Microbiome in Oral Lichen Planus With Desquamative Gingivitis"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4771-2943","authenticated-orcid":false,"given":"Isdee","family":"Jungpraditphol","sequence":"first","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pimchanok","family":"Sutthiboonyapan","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence in Periodontal Disease and Dental Implant Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence in Precision Medicine and Digital Health, Center of Excellence in Genomics and Precision Dentistry, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chompak","family":"Khamwachirapitak","sequence":"additional","affiliation":[{"name":"Center of Excellence in Precision Medicine and Digital Health, Center of Excellence in Genomics and Precision Dentistry, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annop","family":"Krasaesin","sequence":"additional","affiliation":[{"name":"Center of Excellence in Precision Medicine and Digital Health, Center of Excellence in Genomics and Precision Dentistry, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supreda","family":"Srithanyarat","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence in Periodontal Disease and Dental Implant Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence in Precision Medicine and Digital Health, Center of Excellence in Genomics and Precision Dentistry, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0145-9801","authenticated-orcid":false,"given":"Thantrira","family":"Porntaveetus","sequence":"additional","affiliation":[{"name":"Center of Excellence in Precision Medicine and Digital Health, Center of Excellence in Genomics and Precision Dentistry, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Department of Physiology, Geriatric Patients and Special Patients Care International Program, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2340-517X","authenticated-orcid":false,"given":"Paswach","family":"Wiriyakijja","sequence":"additional","affiliation":[{"name":"Center of Excellence in Precision Medicine and Digital Health, Center of Excellence in Genomics and Precision Dentistry, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Department of Oral Medicine, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2006.11.001"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00115\u201023"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/idh.12617"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2017.160745"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201060449\u2010w"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.265645.120"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034519831671"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12935"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep29186"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.4103\/jomfp.JOMFP_304_18"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13232"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915\u2010021\u201001169\u2010z"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201082726\u2010y"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.14142"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2019.05.025"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13323"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13420"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00442"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3290\/j.cjdr.b3086339"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2013.06.027"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866\u2010021\u201002188\u20100"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41385\u2010021\u201000413\u20107"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9995225"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.05.056"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2020.102250"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600\u20100714.2003.00125.x"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2021.1905958"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1195679"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.18502\/fid.v16i1.1103"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.17\u20100721"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3315\/jdcr.2009.1034"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784\u2010024\u201005875\u20100"},{"key":"e_1_2_11_34_1","volume-title":"QIAamp DNA Microbiome Handbook","author":"Technologies, Q. S. a. A","year":"2014"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100714.1992.tb00974.x"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.18\u20100006"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.17\u20100576"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13063"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010024\u201013152\u2010z"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep22943"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00629"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/idj.12607"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13862"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9110952"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.15349","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/odi.15349","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.15349","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T20:39:28Z","timestamp":1769719168000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.15349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,24]]},"references-count":43,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/odi.15349"],"URL":"https:\/\/doi.org\/10.1111\/odi.15349","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/ODI.15349\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.15349\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.15349\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.15349\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.15349\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.15349\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.15349\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.15349\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.15349\/v2\/decision1","asserted-by":"object"}]},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,24]]},"assertion":[{"value":"2024-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}