{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T05:43:16Z","timestamp":1775108596635,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Aim<\/jats:title>\n \n The aim of the present study was to investigate the effects of\n Capnocytophaga periodontitidis<\/jats:italic>\n (\n C.\u2009periodontitidis<\/jats:italic>\n ) and\n \n Porphyromonas gingivalis<\/jats:italic>\n <\/jats:styled-content>\n (\n \n P.\u2009gingivalis<\/jats:italic>\n <\/jats:styled-content>\n ) on dental pulp stem cells (DPSCs) subclusters.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n DPSCs were collected from healthy donors and were infected with\n C.\u2009periodontitidis<\/jats:italic>\n or\n \n P.\u2009gingivalis<\/jats:italic>\n <\/jats:styled-content>\n . High\u2010throughput single\u2010cell RNA sequencing of infected DPSCs was performed to analyze gene expression changes, focusing on differentiation, hypoxia, and cell cycle\u2010related pathways.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Based on our single\u2010cell resolution analysis, four major DPSC clusters were identified: classical\u2010like, fibroblast\u2010like, smooth muscle\u2010like, and perivascular\u2010like.\n C.\u2009periodontitidis<\/jats:italic>\n infection induced differentiation into smooth muscle\u2010like cells, while\n \n P.\u2009gingivalis<\/jats:italic>\n <\/jats:styled-content>\n promoted fibroblast (mineralized) lineages.\n \n P.\u2009gingivalis<\/jats:italic>\n <\/jats:styled-content>\n had a more significant effect on DPSCs differentiation. A specific subcluster responded uniquely to\n C.\u2009periodontitidis<\/jats:italic>\n , with gene expression linked to B cell activation, muscle proliferation, and ROS pathways.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n DPSCs showed the homogeneity and heterogeneity when infected by these two pathogens. DPSCs differentiated into smooth muscle\u2010related cells and mineralization\u2010related cells when infected by\n C.\u2009periodontitidis<\/jats:italic>\n and\n \n P.\u2009gingivalis<\/jats:italic>\n <\/jats:styled-content>\n , respectively. Our results will facilitate understanding and therapeutic strategies of primary periodontal disease with secondary endodontic involvement.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/odi.70045","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T06:29:17Z","timestamp":1753943357000},"page":"216-226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cell Differentiation of\n DPSCs<\/scp>\n Infected With Different Periodontal Pathogens"],"prefix":"10.1111","volume":"32","author":[{"given":"Tiansong","family":"Xu","sequence":"first","affiliation":[{"name":"Central Laboratory Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing China"},{"name":"Fifth Clinical Division Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5255-2040","authenticated-orcid":false,"given":"Xiaofen","family":"Chai","sequence":"additional","affiliation":[{"name":"Central Laboratory Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cariology and Endodontology Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing China"},{"name":"Department of Stomatology Peking University International Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cariology and Endodontology Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Central Laboratory Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2448-6597","authenticated-orcid":false,"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Central Laboratory Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Cariology and Endodontology Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoying","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Cariology and Endodontology Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing China"},{"name":"Center of Stomatology Peking University Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"Central Laboratory Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing 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