{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:17:51Z","timestamp":1787015871839,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T00:00:00Z","timestamp":1755302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T00:00:00Z","timestamp":1755302400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22475006"],"award-info":[{"award-number":["22475006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101041"],"award-info":[{"award-number":["82101041"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7222223"],"award-info":[{"award-number":["7222223"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Synovial macrophages are pivotal regulators of the immune response in temporomandibular joint (TMJ) arthritis. This study investigates Piezo's regulatory effects on macrophage polarization and function in TMJ arthritis progression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A complete Freund's adjuvant (CFA) induced TMJ arthritis model was established in C57BL\/6JNifdc mice, with saline as control. The treated group received Piezo1 inhibitor GsMTx4. Synovitis severity, cartilage degradation, and bone resorption were evaluated by morphological and radiological analyses. Macrophage infiltration, Piezo1, and inflammatory cytokines were analyzed via immunohistochemistry and immunofluorescence. WB and qPCR were used to study signaling pathways in bone marrow\u2010derived macrophages (BMDMs) treated with Piezo1 siRNA, GsMTx4, and Yoda1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Compared to the Saline group, the CFA group exhibited significant infiltration of pro\u2010inflammatory macrophages and prominent osteochondral lesions. Furthermore, IF revealed co\u2010localization of Piezo1, MMP13, MMP3, and TNF\u03b1 with CD86\n                      <jats:sup>+<\/jats:sup>\n                      macrophages. In\u00a0vitro studies demonstrated that Piezo1 activates pro\u2010inflammatory macrophages through the NF\u2010\u03baB pathway, whereas it suppresses anti\u2010inflammatory macrophage polarization by inhibiting the STAT6 pathway. GsMTx4 treatment notably alleviated arthritis symptoms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The disturbed balance between pro\u2010inflammatory and anti\u2010inflammatory macrophages in synovitis may contribute to TMJ arthritis progression. Piezo1 modulates macrophage transcriptional regulation and polarization, influencing immune responses within the synovial environment and offering a potential therapeutic target for TMJ arthritis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70050","type":"journal-article","created":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T18:49:37Z","timestamp":1755370177000},"page":"3126-3139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Piezo1 Modulates Synovial Macrophage Function to Influence the Progression of TMJ Arthritis"],"prefix":"10.1111","volume":"31","author":[{"given":"Lihang","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiliu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2862-1506","authenticated-orcid":false,"given":"Yang","family":"He","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2005.046920"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23482-5"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2020-217363"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.70.6.849"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2004.03.003"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22337"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27290"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.26618"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1193270"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1471-1"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.886604"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.14615"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23683-y"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2006.067470"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2023.11.010"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199910)42:10<2060::AID-ANR6>3.0.CO;2-L"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.715894"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2016.04.010"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2006.02508.x"},{"issue":"6","key":"e_1_2_11_21_1","first-page":"5009","article-title":"Imbalance of M1\/M2 Macrophages Is Linked to Severity Level of Knee Osteoarthritis","volume":"16","author":"Liu B.","year":"2018","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000533525"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2020.12.016"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.123.323223"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2017.01.011"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rehab.2016.01.006"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24044022"},{"issue":"9","key":"e_1_2_11_28_1","article-title":"Association Between Sleep Disorders and Sleep Quality in Patients With Temporomandibular Joint Osteoarthritis: A Systematic Review","volume":"10","author":"S\u00e1nchez R. 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