{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:43:35Z","timestamp":1790678615953,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T00:00:00Z","timestamp":1755302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T00:00:00Z","timestamp":1755302400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Salivary adenoid cystic carcinoma (SACC) frequently metastasizes to the lung and affects patient survival. Extracellular vesicles (EVs) have significant effects in the tumor pre\u2010metastatic niche (PMN). In this study, we aimed to investigate immune changes in the lung pre\u2010metastatic microenvironment of SACC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A pre\u2010metastatic microenvironment was established using SACC\u2010derived EVs at different time points. Labeled EVs were injected into mice to examine their different organ distributions. Flow cytometry was conducted to analyze the immune cell populations. In\u00a0vitro, the same amount of SACC\u2010derived EVs or EV\u2010depleted conditional medium was added to macrophages to stimulate polarization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The SACC\u2010derived EVs colonized mostly in the lung and were engulfed by lung interstitial macrophages (IM). At different time points, the SACC\u2010derived EVs induced decreased total macrophage, natural killer cell, and T cell populations, especially CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cells. They also increased the M2 macrophage composition and regulatory T cell population. The in\u00a0vitro study showed that SACC\u2010derived EVs induce a mixed M1\/M2 phenotype, but this effect was eliminated with the EV\u2010depleted media.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>SACC\u2010derived EVs circulate to the lung, where they are engulfed by IM and induce an immunosuppressive environment. In\u00a0vitro experiments revealed that cargos on EVs could mediate macrophage polarization.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70065","type":"journal-article","created":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T18:29:27Z","timestamp":1755368967000},"page":"142-155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Extracellular Vesicles of Salivary Adenoid Cystic Carcinoma Suppress Immune Microenvironment in Lung"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9092-2605","authenticated-orcid":false,"given":"Yue","family":"Lou","sequence":"first","affiliation":[{"name":"Department of Oral Pathology Peking University School and Hospital of Stomatology and National Center of Stomatology and National Clinical Research Center for Oral Diseases and National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing 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Peking University School and Hospital of Stomatology and National Center of Stomatology and National Clinical Research Center for Oral Diseases and National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology Peking University School and Hospital of Stomatology and National Center of Stomatology and National Clinical Research Center for Oral Diseases and National Engineering Research Center of Oral Biomaterials and Digital Medical Devices  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajuan","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology Peking University School and Hospital of Stomatology and National Center of Stomatology and National Clinical Research Center for Oral Diseases and National Engineering 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