{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T05:42:25Z","timestamp":1776145345727,"version":"3.50.1"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017599","name":"Science and Technology Program of Zhejiang Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017599","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to assess the effect of the low\u2010density lipoprotein receptor\u2010related protein 5 (Lrp5) A241T gain\u2010of\u2010function mutation, which induces a high\u2010bone\u2010mass phenotype, on implant osseointegration in a murine model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Heterozygous Lrp5 A241T mutant (\n                      <jats:italic>Lrp5<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>A\/+<\/jats:italic>\n                      <\/jats:sup>\n                      ) and wild\u2010type (WT) littermate male mice on a C57BL\/6J background were used. Micro\u2010computed tomography (micro\u2010CT) was performed to assess tibial trabecular bone architecture. Sandblasted and acid\u2010etched (SLA) titanium implants were surgically inserted into the proximal tibiae, and samples were harvested after 4\u2009weeks for micro\u2010CT and histological analysis. Primary osteoblasts were seeded onto SLA titanium sheets and induced for osteogenesis for 7\u2009days. Osteogenic marker expression was assessed by quantitative real\u2010time polymerase chain reaction and western blotting.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>Lrp5<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>A\/+<\/jats:italic>\n                      <\/jats:sup>\n                      group exhibited significantly improved trabecular bone architecture compared to WT controls. At 4\u2009weeks post\u2010implantation, micro\u2010CT revealed greater peri\u2010implant bone formation in the\n                      <jats:italic>Lrp5<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>A\/+<\/jats:italic>\n                      <\/jats:sup>\n                      group, while hard tissue sections showed increased bone area and bone\u2010implant contact. HE and Masson staining further confirmed greater new bone formation. After 7\u2009days of in\u00a0vitro osteogenic induction, Lrp5, Runx2, and Sp7 were significantly upregulated in the\n                      <jats:italic>Lrp5<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>A\/+<\/jats:italic>\n                      <\/jats:sup>\n                      group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The Lrp5 A241T mutation significantly enhanced implant osseointegration in a transgenic murine model.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70102","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T10:24:42Z","timestamp":1759227882000},"page":"504-514","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lrp5\n                    <scp>A241T<\/scp>\n          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