{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:23:44Z","timestamp":1788780224138,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To clarify the relationship between oral squamous cell carcinoma (OSCC), potentially malignant oral disease (OPMD), and bacterial flora using metagenomic analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This cross\u2010sectional observational study included 50 patients in the control group and 77 patients with OPMDs, 41 with early OSCCs, and 20 with advanced OSCCs. Patient saliva samples were subjected to high\u2010throughput sequencing of 16S rRNA gene amplicons to evaluate the composition and diversity of the oral microbiome.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      No significant differences were observed in patient backgrounds, other than sex. Patients with advanced OSCCs had greater oral bacterial diversity than those with early OSCC or OPMD. The advanced OSCC group formed a distinct cluster separate from the other groups. Sixteen and 275 species were identified at the phylum and genus levels, respectively. Compared with the control group,\n                      <jats:italic>Actinomycetia<\/jats:italic>\n                      and\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      were significantly elevated in the early OSCC and OPMD groups.\n                      <jats:italic>Peptostreptococcus<\/jats:italic>\n                      and\n                      <jats:italic>Fusobacterium<\/jats:italic>\n                      were significantly higher in the advanced OSCC group than in the control, OPMD, and early OSCC groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The composition and diversity of oral microbiota may be associated with OPMD development and progression to OSCC. Consequently, the salivary microbiome may serve as a biomarker for oral cancer and help predict cancer progression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70115","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T04:30:37Z","timestamp":1760070637000},"page":"992-1003","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Bacterial Involvement in Oral Squamous Cell Carcinoma and Potentially Malignant Oral Disorders"],"prefix":"10.1111","volume":"32","author":[{"given":"Atsumu","family":"Koketsu","sequence":"first","affiliation":[{"name":"Division of Oral and Maxillofacial Oncology and Surgical Sciences, Department of Disease Management Dentistry Tohoku University Graduate School of Dentistry  Sendai Miyagi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Fukase","sequence":"additional","affiliation":[{"name":"Division of 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