{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T19:32:58Z","timestamp":1787859178855,"version":"build-2784847793"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T00:00:00Z","timestamp":1762041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T00:00:00Z","timestamp":1762041600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This observational, cross\u2010sectional, and analytical study aimed to explore the pathophysiological mechanisms of gingivitis in pregnancy by analyzing the salivary proteomic profile according to gingival bleeding status.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Pregnant women at the 27th week of gestation or beyond were categorized into two groups: those with excessive gingival bleeding on probing (BOP &gt;\u200950%; G1) and those without generalized gingivitis (BOP &lt;\u200930%; G2). A comprehensive full\u2010mouth periodontal examination was performed. Unstimulated whole\u2010mouth saliva samples were collected and individually processed using Nano Liquid Chromatography Electron Spray Ionization Tandem Mass Spectrometry (nLC\u2010ESI\u2010MS\/MS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Proteomic analysis identified 187 salivary proteins, with 75 shared between groups. Sixty proteins were upregulated and seven were downregulated in G1. The most upregulated were Protein\n                      <jats:italic>S100\u2010A9<\/jats:italic>\n                      (16\u2010fold),\n                      <jats:italic>Neutrophil Defensins 1<\/jats:italic>\n                      and\n                      <jats:italic>3<\/jats:italic>\n                      (7\u2010fold),\n                      <jats:italic>Protein S100\u2010A8<\/jats:italic>\n                      (5\u2010fold),\n                      <jats:italic>Beta\u20102\u2010Microglobulin<\/jats:italic>\n                      (4\u2010fold), and multiple immunoglobulin isoforms.\n                      <jats:italic>Histatin\u20103<\/jats:italic>\n                      was the only protein downregulated by more than 2\u2010fold. Gene Ontology analysis revealed significant enrichment in processes related to antimicrobial humoral response, bacterial defense mechanisms, and immune regulation, reflecting the inflammatory state.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings provide insights into the salivary proteomic alterations associated with generalized gingivitis in pregnancy, particularly highlighting immune and antimicrobial pathways linked to excessive gingival inflammation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70127","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:05:21Z","timestamp":1762142721000},"page":"852-863","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Salivary Proteomic Signatures in Pregnant Women With Excessive Gingival Bleeding"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4760-8948","authenticated-orcid":false,"given":"Gerson Aparecido","family":"Foratori\u2010Junior","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9208-9562","authenticated-orcid":false,"given":"Clovis Bergamin","family":"Griso","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2598-2228","authenticated-orcid":false,"given":"Amanda Borges","family":"Pirondi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4033-3351","authenticated-orcid":false,"given":"Laura Teodoro","family":"de Marchi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2101-1350","authenticated-orcid":false,"given":"Talita Mendes Oliveira","family":"Ventura","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1469-5224","authenticated-orcid":false,"given":"Larissa Tercilia","family":"Grizzo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5051-1210","authenticated-orcid":false,"given":"Roosevelt","family":"da Silva Bastos","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Orthodontics, and Public Health, Bauru School of Dentistry University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0791-7746","authenticated-orcid":false,"given":"Alejandra","family":"Chaparro","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Centre for Biomedical Research, Faculty of Dentistry Universidad de los Andes  Santiago 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