{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T10:45:54Z","timestamp":1781520354489,"version":"3.54.1"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Although KDM6A plays a pivotal role in various diseases, particularly cancer, the mechanisms underlying its influence on metabolism remain largely elusive. In this study, we aim to reveal a specific post\u2010translational modification of KDM6A to modulate glycolysis in oral squamous cell carcinoma (OSCC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Subjects and Methods<\/jats:title>\n                    <jats:p>\n                      Co\u2010IP and immunoblotting assays were performed to identify KDM6A\u2010pSer829 as a substrate of E3 ubiquitin ligase F\u2010Box and WD Repeat Domain Containing 7 (FBXW7). Phospho\u2010dead\n                      <jats:italic>Kdm6a<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>S829A<\/jats:italic>\n                      <\/jats:sup>\n                      conditional knock\u2010in mice were generated. The tongue tissues from these mice were collected for single\u2010cell RNA sequencing (scRNA\u2010Seq). Using 4\u2010nitroquinoline\u20101\u2010oxide (4NQO) induced OSCC models in mice, the effect of KDM6A\u2010pS829 on cell proliferation was examined by immunofluorescent imaging.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Co\u2010IP and immunoblotting established that KDM6A\u2010pSer829 was a critical FBXW7 recognition site, leading to its ubiquitination and degradation. scRNA\u2010Seq analysis of tongue tissues demonstrated significantly downregulated glycolysis in\n                      <jats:italic>Kdm6a\u2010S829A<\/jats:italic>\n                      mouse oral squamous cells compared to wild\u2010type controls. Furthermore, the\n                      <jats:italic>Kdm6a\u2010S829A<\/jats:italic>\n                      mutation suppressed cell proliferation in 4NQO\u2010induced in\u00a0vivo OSCC models.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings demonstrate that KDM6A\u2010pSer829, targeted by FBXW7 for ubiquitination and degradation, promotes glycolysis and cell proliferation in OSCC. This establishes KDM6A\u2010pSer829 as a critical regulator of OSCC metabolism and tumorigenesis, highlighting its potential as a novel therapeutic target.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70142","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T12:10:05Z","timestamp":1763467805000},"page":"734-744","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Inhibiting\n                    <scp>KDM6A<\/scp>\n                    Phosphorylation Suppresses Glycolysis in Oral Squamous Cell Carcinoma"],"prefix":"10.1111","volume":"32","author":[{"given":"Xin","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head and Neck Oncology, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"National Clinical Research Center for Oral Disease  Shanghai 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Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yikang","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head and Neck Oncology, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"National Clinical Research Center for Oral Disease  Shanghai China"},{"name":"Shanghai Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head and Neck Oncology, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"National Clinical Research Center for Oral Disease  Shanghai China"},{"name":"Shanghai Laboratory of 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