{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T04:05:52Z","timestamp":1784001952938,"version":"3.55.0"},"reference-count":57,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T00:00:00Z","timestamp":1765065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T00:00:00Z","timestamp":1765065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100017950","name":"Shanghai Municipal Health Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Periodontitis is the primary cause of tooth loss worldwide. This study aimed to identify herpes simplex virus (HSV) related biomarkers in periodontitis patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The present study used DIA\u2010based liquid chromatography\u2013tandem mass spectrometry to analyze the proteome of human gingival fibroblasts (HGFs) from one healthy donor across early and late stages (12\u201372\u2009h) of HSV\u20101 infection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The study identified 890 differentially expressed proteins. At the early infection stage, there was a notable upregulation of proteins including interferon (IFN) regulatory factor 7, IFN\u2010stimulated genes 15, interleukin 6 (IL6), toll\u2010like receptor 2 (TLR2), and IFN\u2010induced protein (IFI), alongside a downregulation of matrix metalloproteinase 2 (MMP2). We observed the activation of pathways, including the complement and coagulation cascades, lysosome, nucleotide\u2010binding oligomerization domain\u2010like receptors, retinoic acid\u2010inducible gene I\u2010like receptors, and TLR signaling pathways. Conversely, at the late stage, IFIs, IL1, and MMP3 were significantly upregulated, while complement proteins were downregulated. Biomarkers such as TLR2 may underscore the host's antiviral defense response to HSV\u20101 in the periodontal environment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The present study identified several HGF proteins associated with periodontitis following HSV\u20101 infection, providing an analytical framework for determining the host's anti\u2010viral defense response to antagonize HSV\u20101 infection in periodontal tissues.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70167","type":"journal-article","created":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T19:49:23Z","timestamp":1765136963000},"page":"1399-1411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Inflammatory Cytokine Changes in Herpes Simplex Virus Infected Periodontium: An In\u00a0Vitro Proteomic 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