{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T04:29:17Z","timestamp":1786422557403,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cancer cells remodel blood vessels to create a tumor\u2010supportive environment; however, the spatial distribution of these vessels and their prognostic significance in oral squamous cell carcinoma (OSCC) remain poorly defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We established an open\u2010source pipeline to quantify vascular area (VA) in both the inner tumor (InT) and invasive tumor front (ITF) regions from CD31\u2010stained whole\u2010slide images (discovery cohort,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200966). The associations between VA and patient survival were assessed via univariate and multivariate analyses. Findings were validated in an independent cohort (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200970) immunostained with CD34.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      VA was significantly higher in the InT region compared to the ITF (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0079). A higher VA in the ITF was independently associated with favorable overall survival (discovery: HR 0.28, 95% CI 0.11\u20130.73,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.009; validation: HR 0.18, 95% CI 0.07\u20130.47,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and disease\u2010free survival (discovery: HR 0.34, 95% CI 0.14\u20130.81,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.015; validation: HR 0.25, 95% CI\u00a00.11\u20130.53,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings provide the first evidence that high VA in the ITF is associated with a better prognosis in OSCC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70174","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T19:39:16Z","timestamp":1766432356000},"page":"1571-1579","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tumor Vascular Area Quantification Predicts Survival Outcomes in Oral Squamous Cell Carcinoma"],"prefix":"10.1111","volume":"32","author":[{"given":"Liyuan","family":"Yu","sequence":"first","affiliation":[{"name":"Central Laboratory of Stomatology Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"},{"name":"Department of Oral and Maxillofacial Surgery Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuyan","family":"Li","sequence":"additional","affiliation":[{"name":"Central Laboratory of Stomatology Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"},{"name":"Department of Oral and Maxillofacial Surgery Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingfei","family":"Wang","sequence":"additional","affiliation":[{"name":"Central Laboratory of Stomatology Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"},{"name":"Department of Oral and Maxillofacial Surgery Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2235-3133","authenticated-orcid":false,"given":"Yiwen","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zeng","sequence":"additional","affiliation":[{"name":"State Key Lab of Pharmaceutical Biotechnology, College of Life Sciences Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4522-9664","authenticated-orcid":false,"given":"Runzhi","family":"Deng","sequence":"additional","affiliation":[{"name":"Central Laboratory of Stomatology Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"},{"name":"Department of Oral and Maxillofacial Surgery Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3946-4497","authenticated-orcid":false,"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[{"name":"Central Laboratory of Stomatology Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"},{"name":"Department of Oral and Maxillofacial Surgery Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795-016-0142-7"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.25640"},{"key":"e_1_2_11_4_1","article-title":"Microvascular Density Analysis and Histological Parameters of Oral Cancer Progression","volume":"12","author":"Batistella E. A.","year":"2023","journal-title":"Oral Diseases"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.14694"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/74651"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05696-2"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118746"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32787-y"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32787-y"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.21608\/adjalexu.2019.57362"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.304"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/sonc.2002.37263"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/339058a0"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8081092"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-13-1101"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-21-1059"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1054\/ijom.2003.0433"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5422.1994"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203035"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104819"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2017.06.016"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2012.05.009"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2011.02.011"},{"key":"e_1_2_11_25_1","first-page":"101","article-title":"Tumour Budding in Solid Cancers","volume":"10","author":"Lugli A.","year":"2021","journal-title":"Clinical Oncology"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-020-0422-y"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13402-021-00602-3"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-010-0095-4"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPA.0000000000001237"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm13040976"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.06.020"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3171\/2022.4.FOCUS2210"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1054\/ijom.2002.0284"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2004.00252.x"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21384"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21551"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.5909"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-2391(03)00725-0"},{"issue":"12","key":"e_1_2_11_39_1","first-page":"3403","article-title":"Tumor\u2010Derived Vascular Endothelial Growth Factor Up\u2010Regulates Angiopoietin\u20102 in Host Endothelium and Destabilizes Host Vasculature, Supporting Angiogenesis in Ovarian Cancer","volume":"63","author":"Zhang L.","year":"2003","journal-title":"Cancer Research"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.70174","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/odi.70174","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.70174","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T03:53:57Z","timestamp":1786420437000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.70174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,22]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/odi.70174"],"URL":"https:\/\/doi.org\/10.1111\/odi.70174","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/ODI.70174\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.70174\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.70174\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.70174\/v2\/response1","asserted-by":"object"}]},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,22]]},"assertion":[{"value":"2025-03-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}