{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T16:30:29Z","timestamp":1792773029167,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006182","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e Inova\u00e7\u00e3o do Esp\u00edrito Santo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006182","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To analyze the nuclear abnormalities of cytotoxicity\u2014karyorrhexis (KR), karyolysis (KL), and pyknosis (PN)\u2014and genotoxicity\u2014micronucleus (MN) in exfoliated cells from different sites of the oral mucosa in patients with Epidermolysis Bullosa (EB) and a control group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>This cross\u2010sectional observational study collected sociodemographic and clinical data through physical examination and a standardized questionnaire. Cells from the buccal, labial, and lingual mucosa were obtained via exfoliative cytology and evaluated for MN, KR, KL, and PN frequencies. Mann\u2013Whitney and Kruskal\u2013Wallis tests were applied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 20\u2009EB individuals were selected. The control group (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200920) was matched for sex and age with EB. Males (40%) and females (60%) demonstrated a similar distribution and individuals aged \u2264\u200919\u2009years represented the majority (65%). An increase in KR and KL was detected in EB, with a greater prevalence in the buccal (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0002 and\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001) and labial mucosa (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0005 and\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0240). EB patients showed a higher frequency of MN in the buccal mucosa (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0083).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>An increase in nuclear abnormalities at different sites of the oral mucosa demonstrated the genetic instability associated with EB. Exfoliative cytology represents a potential tool for oral biomonitoring in these patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70316","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T05:05:53Z","timestamp":1776402353000},"page":"2311-2322","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Increased Genetic Instability in Exfoliated Oral Cells in Patients With Epidermolysis Bullosa"],"prefix":"10.1111","volume":"32","author":[{"given":"Ana Carolina Sias Franco","family":"Franzosi","sequence":"first","affiliation":[{"name":"Graduate Program in Dental Sciences Federal University of Esp\u00edrito Santo  Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0402-1480","authenticated-orcid":false,"given":"Rebeca Ferreira","family":"Badar\u00f3","sequence":"additional","affiliation":[{"name":"Graduate Program in Dental Sciences Federal University of Esp\u00edrito Santo  Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Fernandes","family":"Leal","sequence":"additional","affiliation":[{"name":"Faculdades Integradas Esp\u00edrito\u2010Santenses (FAESA) Avenida Ja\u00edr Etienne Dessaune  Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalie Matuk Fuentes","family":"Torrelio","sequence":"additional","affiliation":[{"name":"Hospital Estadual Infantil Nossa Senhora da Gl\u00f3ria Alameda Mari Ubirajara  Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willian Grassi","family":"Bautz","sequence":"additional","affiliation":[{"name":"Department of Morphology Federal University of Esp\u00edrito Santo  Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6330-996X","authenticated-orcid":false,"given":"Leticia Nogueira","family":"da Gama\u2010de\u2010Souza","sequence":"additional","affiliation":[{"name":"Graduate Program in Dental Sciences Federal University of Esp\u00edrito Santo  Vit\u00f3ria Brazil"},{"name":"Department of Morphology Federal University of Esp\u00edrito Santo  Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,16]]},"reference":[{"issue":"2","key":"e_1_2_10_2_1","first-page":"47","article-title":"Micronuclei Assay of Exfoliated Oral Mucosal Cells: A Review","volume":"2","author":"Agarwal M.","year":"2014","journal-title":"Annals of Dental 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