{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T21:59:49Z","timestamp":1785103189687,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K17181"],"award-info":[{"award-number":["18K17181"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K09362"],"award-info":[{"award-number":["23K09362"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Medication\u2010related osteonecrosis of the jaw is a severe adverse effect of antiresorptive agents. Although autologous mesenchymal stromal cell therapy is a potential treatment, the effects of medication\u2010related osteonecrosis of the jaw microenvironment on mesenchymal stromal cells remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mesenchymal stromal cells were isolated from medication\u2010related osteonecrotic jaw lesions of five patients and healthy gingival tissue. Proliferation and differentiation capacities were assessed; transcriptomic profiles were analysed using bulk RNA sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Mesenchymal stromal cells derived from medication\u2010related osteonecrosis of the jaw exhibited proliferation and differentiation capacities comparable to those of gingiva\u2010derived mesenchymal stromal cells. However, substantial transcriptomic alterations in 1336 genes (444 upregulated, 892 downregulated) were identified. Downregulated genes were primarily associated with RNA splicing and processing, including SF3B1 and RBM39. Upregulated genes were enriched in translation, immune responses, and proteoglycan metabolism, including POSTN and FN1, suggesting chronic exposure to medications associated with medication\u2010related osteonecrosis of the jaw induces transcriptomic alterations consistent with disease\u2010associated reprogramming of mesenchymal stromal cells, compromising their regenerative capacity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study provides insights into the applicability and limitations of using mesenchymal stromal cells from patients with medication\u2010related osteonecrosis of the jaw and identifies potential molecular targets for future cell\u2010based regenerative strategies.<\/jats:p>\n                  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