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Comparison with our recently established human embryonic dental cell atlas revealed that epithelium\u2010like cells resembled the most primitive outer enamel progenitors, whereas mesenchyme\u2010like cells did not resemble any embryonic mesenchymal cells, representing a differentiated and abnormal state. Transcription factors\n                      <jats:italic>DLX5<\/jats:italic>\n                      and\n                      <jats:italic>TWIST1<\/jats:italic>\n                      , together with their target genes, showed profound alterations that paralleled the epithelium\u2010to\u2010mesenchyme transition (EMT). We verified the existence of EMT by double immunofluorescence of two EMT markers\n                      <jats:italic>FN1<\/jats:italic>\n                      and\n                      <jats:italic>VIM<\/jats:italic>\n                      , and verified the specific expression of\n                      <jats:italic>KRT15<\/jats:italic>\n                      in pre\u2010marsupialization tissues. Lastly, mesenchyme\u2010derived proteins\n                      <jats:italic>MMP13<\/jats:italic>\n                      and\n                      <jats:italic>THBS2<\/jats:italic>\n                      exert feedback regulatory effects on EMT.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      We constructed the cellular and spatial landscape of conventional ameloblastoma before and after marsupialization. Our findings indicate that the epithelial\u2013mesenchymal transition (EMT) process, potentially regulated by\n                      <jats:italic>DLX5<\/jats:italic>\n                      and\n                      <jats:italic>MMP13<\/jats:italic>\n                      , contributes to the treatment effect of marsupialization.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70422","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T06:34:07Z","timestamp":1784615647000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Single\u2010Cell and Spatial Transcriptomic Analysis of Conventional Ameloblastoma"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8908-9352","authenticated-orcid":false,"given":"Yueqi","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Stomatology Tongren Hospital, Shanghai Jiao 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