{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T05:19:00Z","timestamp":1787635140389,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Periodontitis (PD) is a chronic inflammatory disease characterized by dysregulated immune responses and periodontal tissue destruction. While T lymphocytes are implicated in PD pathogenesis, the roles of specific T cell subsets remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analyzed single\u2010cell RNA sequencing data from periodontal tissues of 5 PD patients and 4 healthy controls (GSE171213). Cell types were annotated using Seurat, and differential expression, transcription factor networks (SCENIC), and cell\u2013cell communication (CellChat) were analyzed to investigate T cell heterogeneity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Thirteen cell types were identified, with a significant reduction in overall T cell proportion in PD. Among T cell subsets, the proportion of CD8 effector T cells was increased in PD patients and exhibited activation of cytotoxic and related inflammatory pathways. Key transcription factors ETV1 and GABPA were upregulated in these CD8 effector T cells from PD patients, regulating target genes including CCL4 and CCL4L2. Cell\u2013cell communication analysis revealed enhanced PARs signaling in PD, mediated by GZMA\u2010F2R and GZMA\u2010PARD3 pairs from CD8 effector T cells to fibroblasts, pericytes, and endothelial cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study suggests that CD8 effector T cells may contribute to PD pathogenesis, potentially through transcriptional reprogramming and amplified PARs signaling, offering new insights into immunopathology and potential therapeutic targets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70423","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T04:26:57Z","timestamp":1787632017000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>scRNA<\/scp>\n                    \u2010Seq Links\n                    <scp>CD8<\/scp>\n                    Effector T Cells to Periodontitis via Transcriptional Reprogramming and Enhanced\n                    <scp>PARs<\/scp>\n                    Signaling"],"prefix":"10.1111","author":[{"given":"Fukai","family":"Ren","sequence":"first","affiliation":[{"name":"Department of Stomatology Changzhi Medical College  Changzhi Shanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiqian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Stomatology Changzhi Medical College  Changzhi Shanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Stomatology Changzhi Medical College  Changzhi Shanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13524"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4096"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13506"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.65694"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12151970"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep09262"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2025.1517154"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184541"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2022.116500"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201045473\u2010y"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13396"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951\u2010023\u201002261\u2010y"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/bsr20210138"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1049\/nbt2.12115"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368\u2010024\u201000291\u20102"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2020.107799"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(19)31146\u20108"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.210.Supp.228.09"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mucimm.2025.02.003"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39857"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.926516"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.4103\/jisp.jisp_287_17"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/4199440"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3290\/j.cjdr.b2440547"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903\u2010015\u20100105\u20108"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1385436"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2025.115253"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.998244"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20100716\u20103040\u20102_12"}],"container-title":["Oral 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