{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:09:26Z","timestamp":1788930566243,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K09922"],"award-info":[{"award-number":["22K09922"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K12969"],"award-info":[{"award-number":["25K12969"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Delayed cervical lymph node metastasis is strongly associated with poor prognosis in early\u2010stage tongue squamous cell carcinoma, but useful biomarkers remain limited. This study evaluated cyclin B1, high\u2010mobility group box protein 1 and 2, and proliferating cell nuclear antigen for stratifying the risk of delayed cervical lymph node metastasis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Eighty\u2010two patients with Stage I\/II tongue squamous cell carcinoma who underwent partial glossectomy without elective neck dissection were included. Cyclin B1 and the cytoplasmic expression of the other three markers were assessed immunohistochemically. Cutoff values were determined using receiver operating characteristic analysis. Cervical lymph node metastasis\u2010free survival was estimated using Kaplan\u2013Meier and multivariable Cox analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Cyclin B1 showed the strongest association with delayed cervical lymph node metastasis (\u03c7\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u200929.0,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001; area under the curve, 0.80; sensitivity, 84.0%; specificity, 77.2%). Dual positivity for cyclin B1 and cytoplasmic high\u2010mobility group box protein 1 showed 76.0% sensitivity and 87.7% specificity and remained significantly associated with metastasis\u2010free survival after adjustment for pathological depth of invasion and Yamamoto\u2013Kohama classification (adjusted hazard ratio, 8.57; 95% confidence interval, 3.44\u201324.36;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Combined cyclin B1 and cytoplasmic high\u2010mobility group box protein 1 assessment may aid postoperative risk stratification.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70476","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T04:57:18Z","timestamp":1788929838000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combined Expression of Cyclin\n                    <scp>B1<\/scp>\n                    and Cytoplasmic\n                    <scp>HMGB1<\/scp>\n                    Is Associated With Delayed Cervical Lymph Node Metastasis in Early\u2010Stage Tongue Squamous Cell Carcinoma"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2697-3188","authenticated-orcid":false,"given":"Wataru","family":"Kakuguchi","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Division of Oral Pathobiological Science, Faculty of Dental Medicine and Graduate School of Dental Medicine Hokkaido University  Sapporo 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