{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T04:34:05Z","timestamp":1793162045722,"version":"4.5.0"},"reference-count":58,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to better understand the expression of P53, CDKN2A, NOTCH1, and CASP8 proteins in progressive and nonprogressive oral epithelial dysplasia (OED) cell lines to predict malignant transformation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Normal oral keratinocytes (NHOK810), nonprogressive OED (D30\u2010mild, D25\u2010severe), progressive OED (D20\u2010moderate, D19\u2010severe), and oral squamous cell carcinoma (H357, BICR31, and ORL188) were co\u2010cultured with irradiated 3T3 fibroblasts. Western blotting was used to assess the protein expression of p53, CDKN2A, NOTCH1, and CASP8 in these cell lines. Bootstrapping, one\u2010way ANOVA, and subsequent post hoc analyses were performed for multiple comparisons.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CDKN2A and NOTCH1 had low expression in progressive OED (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0008 and 0.0012) respectively relative to nonprogressive OED, indicating a negative tendency. Conversely, p53 demonstrated significantly higher expression in progressive OED (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0112) compared to nonprogressive OED, reflecting a positive trend of expression throughout disease progression. There was a significant difference in CASP8 expression between nonprogressive and progressive cell lines (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.039); however, no linear pattern was observed as the disease advanced.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The current study illustrates that the high expression of p53, combined with low expression of CDKN2A and NOTCH1, may serve as helpful markers, providing a potential molecular complement to histopathology for predicting malignant transformation in OED.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.70550","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T04:39:40Z","timestamp":1792471180000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential Expression of\n                    <scp>P53<\/scp>\n                    ,\n                    <scp>CDKN2A<\/scp>\n                    ,\n                    <scp>NOTCH1<\/scp>\n                    , and\n                    <scp>CASP8<\/scp>\n                    in Oral Epithelial Dysplasia: An In\u00a0Vitro Study to Explore Potential Malignant Transformation"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9257-1761","authenticated-orcid":false,"given":"Md. Omar","family":"Faruk","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Clinical Sciences Faculty of Dentistry, Universiti Malaya  Kuala Lumpur Malaysia"},{"name":"Oral Cancer Research &amp; Coordinating Centre (OCRCC), Faculty of Dentistry Universiti Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3511-4791","authenticated-orcid":false,"given":"Lee Peng","family":"Karen\u2010Ng","sequence":"additional","affiliation":[{"name":"Oral Cancer Research &amp; Coordinating Centre (OCRCC), Faculty of Dentistry Universiti Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7061-7378","authenticated-orcid":false,"given":"Wanninayake Mudiyanselage","family":"Tilakaratne","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Clinical Sciences Faculty of Dentistry, Universiti Malaya  Kuala Lumpur Malaysia"},{"name":"Oral Cancer Research &amp; Coordinating Centre (OCRCC), Faculty of Dentistry Universiti Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5986-6170","authenticated-orcid":false,"given":"Thomas George","family":"Kallarakkal","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Clinical Sciences Faculty of Dentistry, Universiti Malaya  Kuala Lumpur Malaysia"},{"name":"Oral Cancer Research &amp; Coordinating Centre (OCRCC), Faculty of Dentistry Universiti Malaya  Kuala Lumpur 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