{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:09:53Z","timestamp":1777439393991,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T00:00:00Z","timestamp":1358985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2013,6]]},"abstract":"Summary<\/jats:title>P<\/jats:styled-content>orphyromonas gingivalis<\/jats:italic> is associated with chronic periodontitis and forms multi\u2010species biofilms. They can communicate within species as well as with other species found in the subgingiva, which may induce changes in the growth ratio and virulence of periodontopathogens. The lipopolysaccharide (LPS<\/jats:styled-content>) of P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> shows different virulence by growth condition. The purpose of this study was to investigate the characteristics of P<\/jats:styled-content>.\u00a0gingivalis <\/jats:italic>LPS<\/jats:styled-content> when co\u2010cultured with F<\/jats:styled-content>usobacterium nucleatum<\/jats:italic>. After culture of P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> in the presence or absence of F<\/jats:styled-content>.\u00a0nucleatum<\/jats:italic>, P<\/jats:styled-content>.\u00a0gingivalis <\/jats:italic>LPS<\/jats:styled-content> was extracted. THP<\/jats:styled-content>\u20101 cells were treated with the LPS<\/jats:styled-content> and induction of cytokine expression was investigated using real\u2010time reverse transcription polymerase chain reaction (RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>) and enzyme\u2010linked immunosorbent assay (ELISA<\/jats:styled-content>). For the analysis of P<\/jats:styled-content>.\u00a0gingivalis <\/jats:italic>LPS<\/jats:styled-content>, LPS<\/jats:styled-content> biosynthesis\u2010related genes such as lpxA<\/jats:italic><\/jats:styled-content> and lpxD<\/jats:italic><\/jats:styled-content> were evaluated with real\u2010time RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>. Finally, molecular mass of lipid A<\/jats:styled-content> was measured by mass spectrometry after hydrolysis of the LPS<\/jats:styled-content>. Co\u2010cultured P<\/jats:styled-content>.\u00a0gingivalis <\/jats:italic>LPS<\/jats:styled-content> exhibited higher induction of expression of interleukin 1\u03b2, 6, and 8 than single\u2010cultured P<\/jats:styled-content>.\u00a0gingivalis <\/jats:italic>LPS<\/jats:styled-content>. These symptoms may be caused by an increase in m<\/jats:italic>\/z<\/jats:italic> 1689 lipid A<\/jats:styled-content> through the upregulation of lpxA<\/jats:italic><\/jats:styled-content> and lpxD<\/jats:italic><\/jats:styled-content> expression by communication between P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> and F<\/jats:styled-content>.\u00a0nucleatum<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/omi.12020","type":"journal-article","created":{"date-parts":[[2012,12,15]],"date-time":"2012-12-15T06:07:24Z","timestamp":1355551644000},"page":"230-238","source":"Crossref","is-referenced-by-count":18,"title":["Characteristics of P<\/scp>orphyromonas gingivalis<\/i> lipopolysaccharide in co\u2010culture with F<\/scp>usobacterium nucleatum<\/i>"],"prefix":"10.1111","volume":"28","author":[{"given":"S.H.","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Oral Microbiology and Immunology College of Dentistry Dankook University Cheonan 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