{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T21:30:41Z","timestamp":1774128641063,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,7,30]],"date-time":"2013-07-30T00:00:00Z","timestamp":1375142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"Summary<\/jats:title>Aggregatibacter actinomycetemcomitans<\/jats:italic> is a Gram\u2010negative bacteria highly associated with localized aggressive periodontitis. The recognition of microbial factors, such as lipopolysaccharide from A<\/jats:styled-content>.\u00a0actinomycetemcomitans<\/jats:italic> (Aa<\/jats:italic><\/jats:sub>LPS<\/jats:styled-content>), in the oral environment is made mainly by surface receptors known as T<\/jats:styled-content>oll\u2010like receptors (TLR<\/jats:styled-content>). TLR<\/jats:styled-content>4 is the major LPS<\/jats:styled-content> receptor. This interaction leads to the production of inflammatory cytokines by myeloid differentiation primary\u2010response protein 88 (M<\/jats:styled-content>yD<\/jats:styled-content>88) \u2010dependent and \u2010independent pathways, which may involve the adaptor T<\/jats:styled-content>oll\/interleukin\u20101 receptor\u2010domain\u2010containing adaptor inducing interferon\u2010\u03b2 (TRIF<\/jats:styled-content>). The aim of this study was to assess the involvement of M<\/jats:styled-content>yD<\/jats:styled-content>88 in alveolar bone loss induced by Aa<\/jats:italic><\/jats:sub>LPS<\/jats:styled-content> in mice. C57BL<\/jats:styled-content>6\/J<\/jats:styled-content> wild\u2010type (WT<\/jats:styled-content>) mice, M<\/jats:styled-content>yD<\/jats:styled-content>88, TRIF<\/jats:styled-content> or TRIF<\/jats:styled-content>\/M<\/jats:styled-content>yD<\/jats:styled-content>88 knockout mice received 10 injections of Aa<\/jats:italic><\/jats:sub>LPS<\/jats:styled-content> strain FDC Y<\/jats:styled-content>4 (5\u00a0\u03bcg in 3\u00a0\u03bcl), in the palatal gingival tissue of the right first molar, every 48\u00a0h. Phosphate\u2010buffered saline was injected in the opposite side and used as control. Animals were sacrificed 24\u00a0h after the 10th injection and the maxillae were removed for macroscopic and biochemical analyses. The injections of Aa<\/jats:italic><\/jats:sub>LPS<\/jats:styled-content> induced significant alveolar bone loss in WT<\/jats:styled-content> mice. In the absence of M<\/jats:styled-content>yD<\/jats:styled-content>88 or TRIF<\/jats:styled-content>\/M<\/jats:styled-content>yD<\/jats:styled-content>88 no bone loss induced by Aa<\/jats:italic><\/jats:sub>LPS<\/jats:styled-content> was observed. In contrast, responses in TRIF<\/jats:styled-content>\u2212\/\u2212<\/jats:sup> mice were similar to those in WT<\/jats:styled-content> mice. Diminished bone loss in the absence of M<\/jats:styled-content>yD<\/jats:styled-content>88 was associated with fewer TRAP<\/jats:styled-content>\u2010positive cells and increased expression of osteoblast markers, RUNX<\/jats:styled-content>2 and osteopontin. There was also reduced tumor necrosis factor\u2010\u03b1 production in M<\/jats:styled-content>yD<\/jats:styled-content>88\u2212\/\u2212<\/jats:sup> mice. There was less osteoclast differentiation of hematopoietic bone marrow cells from M<\/jats:styled-content>yD<\/jats:styled-content>88\u2212\/\u2212<\/jats:sup> mice after Aa<\/jats:italic><\/jats:sub>LPS<\/jats:styled-content> stimulation. Hence, the signaling through M<\/jats:styled-content>yD<\/jats:styled-content>88 is pivotal for Aa<\/jats:italic><\/jats:sub>LPS<\/jats:styled-content>\u2010induced osteoclast formation and alveolar bone loss.<\/jats:p>","DOI":"10.1111\/omi.12034","type":"journal-article","created":{"date-parts":[[2013,7,30]],"date-time":"2013-07-30T08:25:16Z","timestamp":1375172716000},"page":"415-424","source":"Crossref","is-referenced-by-count":34,"title":["MyD<\/scp>88 is essential for alveolar bone loss induced by A<\/scp>ggregatibacter actinomycetemcomitans<\/i> lipopolysaccharide in mice"],"prefix":"10.1111","volume":"28","author":[{"given":"M.F.M.","family":"Madeira","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia ICB Universidade Federal de Minas Gerais (UFMG) Belo Horizonte MG Brazil"},{"name":"Departamento de Bioqu\u00edmica e Imunologia ICB UFMG Belo Horizonte MG 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