{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T17:00:51Z","timestamp":1780592451792,"version":"3.54.1"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,8,14]],"date-time":"2013-08-14T00:00:00Z","timestamp":1376438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Chronic periodontitis is associated with <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>orphyromonas gingivalis<\/jats:italic> infection. Although virulence factors of <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> are hypothesized to contribute to the pathogenesis of periodontitis, it is unclear whether the local <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T\u2010cell\u2010mediated response they elicit prevents or contributes to periodontal bone destruction. We hypothesize that major histocompatibility complex class II I\u2010A<jats:sup>b<\/jats:sup>\u2010binding peptides existing in Kgp and RgpA are presented to <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells during <jats:italic>P.\u00a0gingivalis<\/jats:italic> oral colonization. The protein sequences of gingipains <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>gp<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> and Kgp, and <jats:styled-content style=\"fixed-case\">OMP<\/jats:styled-content>40 and <jats:styled-content style=\"fixed-case\">OMP<\/jats:styled-content>41 of <jats:italic>P.\u00a0gingivalis<\/jats:italic> were scanned using an I\u2010A<jats:sup>b<\/jats:sup>\u2010binding matrix. From this analysis we identified 53 candidate peptides that had the potential to engage the peptide\u2010binding groove of the I\u2010A<jats:sup>b<\/jats:sup> molecule of C57BL\/6 mice. An <jats:styled-content style=\"fixed-case\">ELIS<\/jats:styled-content>pot\u2010based screen revealed those peptide\u2010primed effector\/memory <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells that could be re\u2010stimulated <jats:italic>in vitro<\/jats:italic> with <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> or the peptide itself to produce interleukin\u201017<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> or interferon\u2010\u03b3. Two immunodominant peptides, Kgp<jats:sub>467\u2013477<\/jats:sub> (p<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>gp) and RgpA<jats:sub>1054\u20131064<\/jats:sub>\/Kgp<jats:sub>1074\u20131084<\/jats:sub> (p<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\/Kgp) were identified and engineered to be displayed on I\u2010A<jats:sup>b<\/jats:sup> molecular tetramers. Peptide p<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\/Kgp is conserved across all sequenced <jats:italic>P.\u00a0gingivalis<\/jats:italic> strains. <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice were orally inoculated with <jats:italic>P.\u00a0gingivalis<\/jats:italic> strain 53977 and cervical lymph node cells were stained with phycoerythrin\u2010conjugated p<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>gp::I\u2010A<jats:sup>b<\/jats:sup> and p<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>gp::I\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content><jats:sup>b<\/jats:sup> tetramers. We found that only p<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\/Kgp::I\u2010A<jats:sup>b<\/jats:sup> bound with the desired specificity to gingipain\u2010specific <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells. The p<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\/Kgp::I\u2010A<jats:sup>b<\/jats:sup> tetramer complex will allow the identification of effector\/memory <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells specific for two virulence factors of <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> strains associated with periodontal disease.<\/jats:p>","DOI":"10.1111\/omi.12038","type":"journal-article","created":{"date-parts":[[2013,8,14]],"date-time":"2013-08-14T12:16:09Z","timestamp":1376482569000},"page":"452-466","source":"Crossref","is-referenced-by-count":18,"title":["Identification of gingipain\u2010specific <scp>I<\/scp>\u2010<scp>A<\/scp><sup>b<\/sup>\u2010restricted <scp>CD<\/scp>4<sup>+<\/sup><scp>T<\/scp> cells following mucosal colonization with <i><scp>P<\/scp>orphyromonas gingivalis<\/i> in <scp>C<\/scp>57<scp>BL<\/scp>\/6 mice"],"prefix":"10.1111","volume":"28","author":[{"given":"P.D.","family":"Bittner\u2010Eddy","sequence":"first","affiliation":[{"name":"Division of Periodontology Department of Developmental and Surgical Sciences School of Dentistry University of Minnesota Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.A.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Division of Periodontology Department of Developmental and Surgical Sciences School of Dentistry University of Minnesota Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Costalonga","sequence":"additional","affiliation":[{"name":"Division of Periodontology Department of Developmental and Surgical Sciences School of Dentistry University of Minnesota Minneapolis MN 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