{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T10:32:44Z","timestamp":1789813964400,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,5,10]],"date-time":"2014-05-10T00:00:00Z","timestamp":1399680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100007877","name":"Fujita Health University","doi-asserted-by":"crossref","award":["No Numbered (2013)"],"award-info":[{"award-number":["No Numbered (2013)"]}],"id":[{"id":"10.13039\/501100007877","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Porphyromonas gingivalis<\/jats:italic> is a keystone periodontal pathogen. Histologocally, the gingival tissue in periodontitis shows dense infiltration of plasma cells. However, antigens recognized by antibodies secreted from the immunocytes remain unknown. The enzyme\u2010labeled antigen method was applied to detecting plasma cells producing <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic>\u2010specific antibodies in biopsied gingival tissue of periodontitis. <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010terminally biotinylated <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> antigens, <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g53 and four gingipain domains (<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rg\u2010pro, <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rg\u2010hgp, <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>ys\u2010pro and <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>ys\u2010hgp) were prepared by the cell\u2010free protein synthesis system using wheatgerm extract. With these five labeled proteins as probes, 20 lesions of periodontitis were evaluated. With the <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>lpha<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>creen method, antibodies against any one of the five <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> antigens were detected in 11 (55%) serum samples and 17 (85%) tissue extracts. Using the enzyme\u2010labeled antigen method on paraformaldehyde\u2010fixed frozen sections of gingival tissue, plasma cells were labeled with any one of the five antigens in 17 (94%) of 18 specimens, in which evaluable plasma cells were detected. The positivity rates in periodontitis were significantly higher than those found previously in radicular cysts (20% in sera and 33% in tissue extracts with the <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>lpha<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>creen method, and 25% with the enzyme\u2010labeled antigen method). Our findings directly indicate that antibodies reactive to <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> are locally produced in the gingival lesions, and that inflammatory reactions against <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> are involved in periodontitis.<\/jats:p>","DOI":"10.1111\/omi.12052","type":"journal-article","created":{"date-parts":[[2014,4,2]],"date-time":"2014-04-02T14:54:53Z","timestamp":1396450493000},"page":"156-173","source":"Crossref","is-referenced-by-count":16,"title":["<i>In situ<\/i> visualization of plasma cells producing antibodies reactive to <i><scp>P<\/scp>orphyromonas gingivalis<\/i> in periodontitis: the application of the enzyme\u2010labeled antigen method"],"prefix":"10.1111","volume":"29","author":[{"given":"Y.","family":"Mizutani","sequence":"first","affiliation":[{"name":"Department of Pathology Fujita Health University School of Medicine Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Tsuge","sequence":"additional","affiliation":[{"name":"Department of Pathology Fujita Health University School of Medicine Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Takeda","sequence":"additional","affiliation":[{"name":"Division of Cell\u2010Free Sciences Proteo\u2010Science Center Ehime University Matsuyama Ehime Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Pathology Fujita Health University School of Medicine Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Shiogama","sequence":"additional","affiliation":[{"name":"Department of Pathology Fujita Health University School of Medicine Toyoake Aichi 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