{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:36:13Z","timestamp":1761489373761},"reference-count":56,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,6,3]],"date-time":"2014-06-03T00:00:00Z","timestamp":1401753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Treponema denticola<\/jats:italic>, a periopathogen, evades complement\u2010mediated killing by binding the negative complement regulatory protein factor H (<jats:styled-content style=\"fixed-case\">FH<\/jats:styled-content>) to its surface via the FhbB protein. Paradoxically, bound <jats:styled-content style=\"fixed-case\">FH<\/jats:styled-content> is cleaved by <jats:italic>T.\u00a0denticola<\/jats:italic>'s dentilisin protease, a process hypothesized to trigger localized dysregulation of complement activation in periodontal pockets. The ability of other oral treponemes to evade complement\u2010mediated killing and bind and cleave <jats:styled-content style=\"fixed-case\">FH<\/jats:styled-content> has not been assessed. In this report, we demonstrate that representative isolates of <jats:italic>Treponema socranskii<\/jats:italic>,<jats:italic> Treponema medium<\/jats:italic>,<jats:italic> Treponema pectinovorum<\/jats:italic> and <jats:italic>Treponema maltophilum<\/jats:italic> are also serum resistant, whereas <jats:italic>Treponema vincentii<\/jats:italic> and <jats:italic>Treponema amylovorum<\/jats:italic> are serum sensitive. Although <jats:italic>T.\u00a0denticola<\/jats:italic>'s ability to evade complement\u2010mediated killing is strictly dependent on <jats:styled-content style=\"fixed-case\">FH<\/jats:styled-content> binding, other serum\u2010resistant treponemal species lack <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>hb<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> and do not bind <jats:styled-content style=\"fixed-case\">FH<\/jats:styled-content>, indicating an <jats:styled-content style=\"fixed-case\">FH<\/jats:styled-content>\u2010independent mechanism of complement evasion. To assess the influence of FhbB sequence variation on <jats:styled-content style=\"fixed-case\">FH<\/jats:styled-content> binding and cleavage by <jats:italic>T.\u00a0denticola<\/jats:italic>,<jats:italic> fhbB<\/jats:italic> sequences were determined for 30 isolates. Three distinct phyletic types were identified. All <jats:italic>T.\u00a0denticola<\/jats:italic> strains bound <jats:styled-content style=\"fixed-case\">FH<\/jats:styled-content> and were serum resistant, but differences in binding kinetics, dentilisin activity and <jats:styled-content style=\"fixed-case\">FH<\/jats:styled-content> cleavage ability were observed. Based on these analyses, we hypothesize that the composition of the <jats:italic>T.\u00a0denticola<\/jats:italic> population is a determining factor that influences the progression and severity of periodontal disease.<\/jats:p>","DOI":"10.1111\/omi.12054","type":"journal-article","created":{"date-parts":[[2014,5,10]],"date-time":"2014-05-10T15:08:43Z","timestamp":1399734523000},"page":"194-207","source":"Crossref","is-referenced-by-count":19,"title":["Analysis of the complement sensitivity of oral treponemes and the potential influence of <scp>FH<\/scp> binding, <scp>FH<\/scp> cleavage and dentilisin activity on the pathogenesis of periodontal disease"],"prefix":"10.1111","volume":"29","author":[{"given":"D.P.","family":"Miller","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Medical College of Virginia Virginia Commonwealth University Richmond VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.V.","family":"McDowell","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Medical College of Virginia Virginia Commonwealth University Richmond VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.K.","family":"Bell","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Medical College of Virginia Virginia Commonwealth University Richmond VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.P.","family":"Goetting\u2010Minesky","sequence":"additional","affiliation":[{"name":"Department of Biologic and Materials Sciences School of Dentistry University of Michigan Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.C.","family":"Fenno","sequence":"additional","affiliation":[{"name":"Department of Biologic and Materials Sciences School of Dentistry University of Michigan Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.T.","family":"Marconi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Medical College of Virginia Virginia Commonwealth University Richmond VA USA"},{"name":"Center for the Study of Biological Complexity Medical College of Virginia Virginia Commonwealth University Richmond VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,6,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq399"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00258-07"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01310-08"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.323287"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003470"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.2.1252-1255.2005"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411910020030401"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345780570010101"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2003.08.001"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2337"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh398"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00108.x"},{"key":"e_1_2_6_14_1","first-page":"581","article-title":"Insertional inactivation of the prtP gene of Treponema denticola confirms dentilisin's disruption of epithelial junctions","volume":"2","author":"Ellen R.P.","year":"2000","journal-title":"J Mol Microbiol Biotech"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2010.05.007"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5769(00)00040-0"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200323541"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12013"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000698"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.9.2612-2618.2004"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2007-2014.2006"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01266-07"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.09R23FM"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.12.5178-5186.1996"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.15.3837-3844.1998"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2007.12.075"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ebd.6400581"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7126-7132.2005"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01458-06"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01544-08"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2010.00598.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2009.103"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309111011298"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.339721"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12027"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2462-2467.2006"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.8.5537"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071069"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1923"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.11.7292"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-4310(95)91170-7"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340230306"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1977.48.12.772"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1977.48.12.778"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053659"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2011.75"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07373.x"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2008.01442.x"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.10.2717-2722.1988"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05604-11"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.02.013"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2620"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0300971"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.06.149"}],"container-title":["Molecular Oral Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fomi.12054","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/omi.12054","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T18:50:22Z","timestamp":1696186222000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/omi.12054"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,3]]},"references-count":56,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1111\/omi.12054"],"URL":"https:\/\/doi.org\/10.1111\/omi.12054","archive":["Portico"],"relation":{},"ISSN":["2041-1006","2041-1014"],"issn-type":[{"value":"2041-1006","type":"print"},{"value":"2041-1014","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,3]]}}}