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Envelope protein composition is influenced by the physiological and environmental demands placed on the bacterium. In this study, we report a comprehensive compilation of cell envelope proteins from the periodontal and systemic pathogen <jats:italic>Aggregatibacter actinomycetemcomitans <\/jats:italic><jats:styled-content style=\"fixed-case\">VT<\/jats:styled-content>1169, an afimbriated serotype b strain. The urea\u2010extracted membrane proteins were identified by mass spectrometry\u2010based shotgun proteomics. The membrane proteome, isolated from actively growing bacteria under normal laboratory conditions, included 648 proteins representing 27% of the predicted open reading frames in the genome. Bioinformatic analyses were used to annotate and predict the cellular location and function of the proteins. Surface adhesins, porins, lipoproteins, numerous influx and efflux pumps, multiple sugar, amino acid and iron transporters, and components of the type I, <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> and V secretion systems were identified. Periplasmic space and cytoplasmic proteins with chaperone function were also identified. A total of 107 proteins with unknown function were associated with the cell envelope. Orthologs of a subset of these uncharacterized proteins are present in other bacterial genomes, whereas others are found exclusively in <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic>. This knowledge will contribute to elucidating the role of cell envelope proteins in bacterial growth and survival in the oral cavity.<\/jats:p>","DOI":"10.1111\/omi.12074","type":"journal-article","created":{"date-parts":[[2014,7,23]],"date-time":"2014-07-23T15:27:22Z","timestamp":1406129242000},"page":"97-110","source":"Crossref","is-referenced-by-count":10,"title":["The cell envelope proteome of <i>Aggregatibacter actinomycetemcomitans<\/i>"],"prefix":"10.1111","volume":"30","author":[{"given":"K.P.","family":"Smith","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics University of Vermont Burlington VT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.G.","family":"Fields","sequence":"additional","affiliation":[{"name":"Department of Biology University of Vermont Burlington VT USA"},{"name":"Vermont Genetics Network Proteomics Facility University of Vermont Burlington VT 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