{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T18:11:10Z","timestamp":1783534270269,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,11,10]],"date-time":"2014-11-10T00:00:00Z","timestamp":1415577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"a Grant-in Aid for Scientific Research (B) from the Japan Society for the Promotion of Science","award":["2339047315"],"award-info":[{"award-number":["2339047315"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2015,6]]},"abstract":"Summary<\/jats:title>Glucan\u2010binding proteins (G<\/jats:styled-content>bps) of S<\/jats:styled-content>treptococcus mutans<\/jats:italic>, a major pathogen of dental caries, mediate the binding of glucans synthesized from sucrose by the action of glucosyltransferases (GTF<\/jats:styled-content>s) encoded by gtfB<\/jats:styled-content><\/jats:italic>, gtfC<\/jats:styled-content><\/jats:italic>, and gtfD<\/jats:styled-content><\/jats:italic>. Several stress proteins, including D<\/jats:styled-content>naK<\/jats:styled-content> and G<\/jats:styled-content>roEL<\/jats:styled-content> encoded by dnaK<\/jats:styled-content><\/jats:italic> and groEL<\/jats:styled-content><\/jats:italic>, are related to environmental stress tolerance. The contribution of G<\/jats:styled-content>bp expression to biofilm formation was analyzed by focusing on the expression levels of genes encoding GTF<\/jats:styled-content>s and stress proteins. Biofilm\u2010forming assays were performed using G<\/jats:styled-content>bpA<\/jats:styled-content>\u2010, G<\/jats:styled-content>bpB<\/jats:styled-content>\u2010, and G<\/jats:styled-content>bpC<\/jats:styled-content>\u2010deficient mutant strains and the parental strain MT<\/jats:styled-content>8148. The expression levels of gtfB<\/jats:styled-content><\/jats:italic>, gtfC<\/jats:styled-content><\/jats:italic>, gtfD<\/jats:styled-content><\/jats:italic>, dnaK<\/jats:styled-content><\/jats:italic>, and groEL<\/jats:styled-content><\/jats:italic> were evaluated by reverse transcription\u2010quantitative polymerase chain reaction (RT<\/jats:styled-content>\u2010qPCR<\/jats:styled-content>). Furthermore, the structure of biofilms formed by these G<\/jats:styled-content>bp\u2010deficient mutant strains was observed using confocal laser scanning microscopy (CLSM<\/jats:styled-content>). Biofilm\u2010forming assay findings demonstrated that the amount formed by the G<\/jats:styled-content>bpA<\/jats:styled-content>\u2010deficient mutant strain (AD<\/jats:styled-content>1) was nearly the same as that by the parental strain, while the G<\/jats:styled-content>bpB<\/jats:styled-content>\u2010 and G<\/jats:styled-content>bpC<\/jats:styled-content>\u2010deficient mutant strains produced lower amounts than MT<\/jats:styled-content>8148. Furthermore, RT<\/jats:styled-content>\u2010qPCR<\/jats:styled-content> assay results showed that the expressions of gtfB<\/jats:styled-content><\/jats:italic>, dnaK<\/jats:styled-content><\/jats:italic>, and groEL<\/jats:styled-content><\/jats:italic> in AD<\/jats:styled-content>1 were elevated compared with MT<\/jats:styled-content>8148. CLSM<\/jats:styled-content> also revealed that the structure of biofilm formed by AD<\/jats:styled-content>1 was prominently different compared with that formed by the parental strain. These results suggest that a defect in G<\/jats:styled-content>bpA<\/jats:styled-content> influences the expression of genes controlling biofilm formation, indicating its importance as a protein for firm and stable biofilm formation.<\/jats:p>","DOI":"10.1111\/omi.12085","type":"journal-article","created":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T13:52:10Z","timestamp":1411653130000},"page":"217-226","source":"Crossref","is-referenced-by-count":25,"title":["Contribution of glucan\u2010binding protein A<\/scp> to firm and stable biofilm formation by S<\/scp>treptococcus mutans<\/i>"],"prefix":"10.1111","volume":"30","author":[{"given":"Y.","family":"Matsumi","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Takashima","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Yanagida","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Morikawa","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Matsumoto\u2010Nakano","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama 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