{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T14:43:04Z","timestamp":1775486584004,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,7,2]],"date-time":"2015-07-02T00:00:00Z","timestamp":1435795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The development of dental caries and periodontal diseases result from distinct shifts in the microbiota of the tooth\u2010associated biofilm. This <jats:italic>in\u00a0vitro<\/jats:italic> study aimed to investigate changes in biofilm composition and structure, during the shift from a \u2018supragingival\u2019 aerobic profile to a \u2018subgingival\u2019 anaerobic profile. Biofilms consisting of <jats:italic>Actinomyces oris<\/jats:italic>,<jats:italic> Candida albicans<\/jats:italic>,<jats:italic> Fusobacterium nucleatum<\/jats:italic>,<jats:italic> Streptococcus oralis<\/jats:italic>,<jats:italic> Streptococcus mutans<\/jats:italic> and <jats:italic>Veillonella dispar<\/jats:italic> were aerobically grown in saliva\u2010containing medium on hydroxyapatite disks. After 64\u00a0h, <jats:italic>Campylobacter rectus<\/jats:italic>,<jats:italic> Prevotella intermedia<\/jats:italic> and <jats:italic>Streptococcus anginosus<\/jats:italic> were further added along with human serum, while culture conditions were shifted to microaerophilic. After 96\u00a0h, <jats:italic>Porphyromonas gingivalis<\/jats:italic>,<jats:italic> Tannerella forsythia<\/jats:italic> and <jats:italic>Treponema denticola<\/jats:italic> were finally added and the biofilm was grown anaerobically for another 64\u00a0h. At the end of each phase, biofilms were harvested for species\u2010specific quantification and localization. Apart from <jats:italic>C.\u00a0albicans<\/jats:italic>, all other species gradually increased during aerobic and microaerophilic conditions, but remained steady during anaerobic conditions. Biofilm thickness was doubled during the microaerophilic phase, but remained steady throughout the anaerobic phase. Extracellular polysaccharide presence was gradually reduced throughout the growth period. Biofilm viability was reduced during the microaerophilic conversion, but was recovered during the anaerobic phase. This <jats:italic>in\u00a0vitro<\/jats:italic> study has characterized the\u00a0dynamic structural shifts occurring in an oral biofilm model during the switch from aerobic to anaerobic conditions, potentially modeling the conversion of supragingival to subgingival biofilms. Within the limitations of this experimental model, the findings may provide novel insights into the ecology of oral biofilms.<\/jats:p>","DOI":"10.1111\/omi.12108","type":"journal-article","created":{"date-parts":[[2015,5,29]],"date-time":"2015-05-29T19:15:22Z","timestamp":1432926922000},"page":"125-135","source":"Crossref","is-referenced-by-count":46,"title":["Microbial dynamics during conversion from supragingival to subgingival biofilms in an <i>in\u00a0vitro<\/i> model"],"prefix":"10.1111","volume":"31","author":[{"given":"T.","family":"Thurnheer","sequence":"first","affiliation":[{"name":"Oral Microbiology and Immunology Institute of Oral Biology Center of Dental Medicine University of Z\u00fcrich Z\u00fcrich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Bostanci","sequence":"additional","affiliation":[{"name":"Oral Microbiology and Immunology Institute of Oral Biology Center of Dental Medicine University of Z\u00fcrich Z\u00fcrich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.N.","family":"Belibasakis","sequence":"additional","affiliation":[{"name":"Oral Microbiology and Immunology Institute of Oral Biology Center of Dental Medicine University of Z\u00fcrich Z\u00fcrich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5721-5732.2005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03857.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083090"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12034"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2013.130167"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2011.03.009"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.30.s5.3.x"},{"key":"e_1_2_7_9_1","first-page":"279","volume-title":"Use of Continuous Flow Techniques in Modeling Dental Plaque Biofilms","author":"Bradshaw D.J.","year":"1999"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.5.3678-3684.2006"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000329669"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031092"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-43-4-631"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509351812"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1128\/iai.42.2.459-470.1983","article-title":"Antigenic heterogeneity of Bacteroides intermedius as recognized by monoclonal antibodies","volume":"42","author":"Gm\u00fcr R.","year":"1983","journal-title":"Infect Immun"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11940-003-0019-7"},{"key":"e_1_2_7_17_1","first-page":"89","article-title":"Enzymatic hydrolysis and structure of water\u2010insoluble glucan produced by glucosyltransferases from a strain of Streptococcus mutans","volume":"14","author":"Guggenheim B.","year":"1970","journal-title":"Helv Odont Acta"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345010800011201"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.3.1343-1350.2001"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-280"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2008.07.012"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1991.tb01730.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058825"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2014.969249"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2003.01627.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.175.11.3247-3252.1993"},{"key":"e_1_2_7_27_1","first-page":"322","volume-title":"Spatial Organization of Oral Bacteria in Biofilms","author":"Kolenbrander P.E.","year":"1999"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.027953-0"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12067"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01569826"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6831-6-S1-S14"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01673.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02483.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00740-06"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1159\/000057866"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.7188-7192.2003"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.4161\/21505594.2014.967608"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00226-8"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.3.1702-1709.2003"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2003.09.003"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1159\/000091065"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2014.05.023"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-013-1157-2"},{"key":"e_1_2_7_45_1","first-page":"239","article-title":"Effects of combined exogenous dextranase and sodium fluoride on Streptococcus mutans 25175 monospecies biofilms","volume":"26","author":"Yang Y.M.","year":"2013","journal-title":"Am J Dent"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009321"}],"container-title":["Molecular Oral Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fomi.12108","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/omi.12108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T16:38:17Z","timestamp":1696178297000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/omi.12108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,2]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.1111\/omi.12108"],"URL":"https:\/\/doi.org\/10.1111\/omi.12108","archive":["Portico"],"relation":{},"ISSN":["2041-1006","2041-1014"],"issn-type":[{"value":"2041-1006","type":"print"},{"value":"2041-1014","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,2]]}}}