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Despite their biological importance, the mechanisms of\n                    <jats:styled-content style=\"fixed-case\">OMV<\/jats:styled-content>\n                    biogenesis have not been fully elucidated. The ~14 times more curvature of the\n                    <jats:styled-content style=\"fixed-case\">OMV<\/jats:styled-content>\n                    membrane than cell outer membrane (\n                    <jats:styled-content style=\"fixed-case\">OM<\/jats:styled-content>\n                    ) indicates that\n                    <jats:styled-content style=\"fixed-case\">OMV<\/jats:styled-content>\n                    biogenesis requires energy expenditure for significant curvature of the\n                    <jats:styled-content style=\"fixed-case\">OMV<\/jats:styled-content>\n                    membrane. In\n                    <jats:italic>P.\u00a0gingivalis<\/jats:italic>\n                    , we propose that this may be achieved by upregulating the production of certain inner or outer leaflet lipids, which causes localized outward curvature of the\n                    <jats:styled-content style=\"fixed-case\">OM<\/jats:styled-content>\n                    . This results in selection of anionic lipopolysaccharide (A\u2010\n                    <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\n                    ) and associated C\u2010terminal domain (\n                    <jats:styled-content style=\"fixed-case\">CTD<\/jats:styled-content>\n                    ) \u2010family proteins on the outer surface due to their ability to accommodate the curvature. Deacylation of A\u2010\n                    <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\n                    may further enable increased curvature leading to\n                    <jats:styled-content style=\"fixed-case\">OMV<\/jats:styled-content>\n                    formation.\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    <jats:styled-content style=\"fixed-case\">OMV<\/jats:styled-content>\n                    s that are selectively enriched in\n                    <jats:styled-content style=\"fixed-case\">CTD<\/jats:styled-content>\n                    \u2010family proteins, largely the gingipains, can support bacterial coaggregation, promote biofilm development and act as an intercessor for the transport of non\u2010motile bacteria by motile bacteria. The\n                    <jats:italic>P.\u00a0gingivalis<\/jats:italic>\n                    <jats:styled-content style=\"fixed-case\">OMV<\/jats:styled-content>\n                    s are also believed to contribute to host interaction and colonization, evasion of immune defense mechanisms, and destruction of periodontal tissues. They may be crucial for both micro\u2010 and macronutrient capture, especially heme and probably other assimilable compounds for its own benefit and that of the wider biofilm community.\n                  <\/jats:p>","DOI":"10.1111\/omi.12134","type":"journal-article","created":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T04:16:57Z","timestamp":1441858617000},"page":"365-378","source":"Crossref","is-referenced-by-count":142,"title":["Spheres of influence:\n                    <i>Porphyromonas gingivalis<\/i>\n                    outer membrane vesicles"],"prefix":"10.1111","volume":"31","author":[{"given":"M.J.","family":"Gui","sequence":"first","affiliation":[{"name":"Oral Health Cooperative Research Centre Melbourne Dental School Bio21 Molecular Science and Biotechnology Institute University of Melbourne Victoria Vic Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.G.","family":"Dashper","sequence":"additional","affiliation":[{"name":"Oral Health 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