{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T20:40:17Z","timestamp":1788986417795,"version":"build-2803163510"},"reference-count":71,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,11,23]],"date-time":"2015-11-23T00:00:00Z","timestamp":1448236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005277","name":"Presbyterian Historical Society","doi-asserted-by":"publisher","award":["DE022465"],"award-info":[{"award-number":["DE022465"]}],"id":[{"id":"10.13039\/100005277","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005277","name":"Presbyterian Historical Society","doi-asserted-by":"publisher","award":["DE006014"],"award-info":[{"award-number":["DE006014"]}],"id":[{"id":"10.13039\/100005277","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005277","name":"Presbyterian Historical Society","doi-asserted-by":"publisher","award":["DE09517"],"award-info":[{"award-number":["DE09517"]}],"id":[{"id":"10.13039\/100005277","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Porphyromonas gingivalis<\/jats:italic> often subverts host cell autophagic processes for its own survival. Our previous studies document the association of the cargo sorting protein, melanoregulin (MREG), with its binding partner, the autophagic protein, microtubule\u2010associated protein 1 light chain 3 (LC3) in macrophages incubated with <jats:italic>P.\u00a0gingivalis<\/jats:italic> (strain 33277). Differences in the lipid A moiety of lipopolysaccharide (LPS) affect the virulence of <jats:italic>P.\u00a0gingivalis<\/jats:italic>; penta\u2010acylated LPS<jats:sub>1690<\/jats:sub> is a weak Toll\u2010like receptor 4 agonist compared with <jats:italic>Escherichia coli<\/jats:italic> LPS, whereas tetra\u2010acylated LPS<jats:sub>1435\/1449<\/jats:sub> acts as an LPS<jats:sub>1690<\/jats:sub> antagonist. To determine how <jats:italic>P.\u00a0gingivalis<\/jats:italic> LPS<jats:sub>1690<\/jats:sub> affects autophagy we assessed LC3\u2010dependent and MREG\u2010dependent processes in green fluorescent protein (GFP)\u2010LC3\u2010expressing Saos\u20102 cells. LPS<jats:sub>1690<\/jats:sub> stimulated the formation of very large LC3\u2010positive vacuoles and MREG puncta. This LPS<jats:sub>1690<\/jats:sub>\u2010mediated LC3 lipidation decreased in the presence of LPS<jats:sub>1435\/1449<\/jats:sub>. When Saos\u20102 cells were incubated with <jats:italic>P.\u00a0gingivalis<\/jats:italic> the bacteria internalized but did not traffic to GFP\u2010LC3\u2010positive structures. Nevertheless, increases in LC3 lipidation and MREG puncta were observed. Collectively, these results suggest that <jats:italic>P.\u00a0gingivalis<\/jats:italic> internalization is not necessary for LC3 lipidation. Primary human gingival epithelial cells isolated from patients with periodontitis showed both LC3II and MREG puncta whereas cells from disease\u2010free individuals exhibited little co\u2010localization of these two proteins. These results suggest that the prevalence of a particular LPS moiety may modulate the degradative capacity of host cells, so influencing bacterial survival.<\/jats:p>","DOI":"10.1111\/omi.12141","type":"journal-article","created":{"date-parts":[[2015,10,9]],"date-time":"2015-10-09T12:40:48Z","timestamp":1444394448000},"page":"486-500","source":"Crossref","is-referenced-by-count":22,"title":["Variants of <i>Porphyromonas gingivalis<\/i> lipopolysaccharide alter lipidation of autophagic protein, microtubule\u2010associated protein 1 light chain 3, LC3"],"prefix":"10.1111","volume":"31","author":[{"given":"I.","family":"Blasi","sequence":"first","affiliation":[{"name":"Department of Orthodontics School of Dental Medicine University of Pennsylvania Philadelphia PA USA"},{"name":"Department of Orthodontics School of Dentistry International University of Catalonia Barcelona 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