{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:01:24Z","timestamp":1791460884516,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,2,4]],"date-time":"2016-02-04T00:00:00Z","timestamp":1454544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001637","name":"Trinity College Dublin","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001637","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004330","name":"GlaxoSmithKline","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004330","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    is a bacterium associated with chronic periodontitis that possesses a family of genes encoding hemagglutinins required for heme acquisition. In this study we generated \u0394\n                    <jats:italic>hagB<\/jats:italic>\n                    and \u0394\n                    <jats:italic>hagC<\/jats:italic>\n                    mutants in strain W83 and demonstrate that both\n                    <jats:italic>hagB<\/jats:italic>\n                    and\n                    <jats:italic>hagC<\/jats:italic>\n                    are required for adherence to oral epithelial cells. Unexpectedly, a double \u0394\n                    <jats:italic>hagB<\/jats:italic>\n                    \/\u0394\n                    <jats:italic>hagC<\/jats:italic>\n                    mutant had less severe adherence defects than either of the single mutants, but was found to exhibit increased expression of the gingipain\u2010encoding genes\n                    <jats:italic>rgpA<\/jats:italic>\n                    and\n                    <jats:italic>kgp<\/jats:italic>\n                    , suggesting that a \u0394\n                    <jats:italic>hagB<\/jats:italic>\n                    \/\u0394\n                    <jats:italic>hagC<\/jats:italic>\n                    mutant is only viable in populations of cells that exhibit increased expression of genes involved in heme acquisition. Disruption of\n                    <jats:italic>hagB<\/jats:italic>\n                    in the fimbriated strain ATCC33277 demonstrated that HagB is also required for stable attachment of fimbriated bacteria to oral epithelial cells. Mutants of\n                    <jats:italic>hagC<\/jats:italic>\n                    were also found to form defective single and multi\u2010species biofilms that had reduced biomass relative to biofilms formed by the wild\u2010type strain. This study highlights the hitherto unappreciated importance of these genes in oral colonization and biofilm formation.\n                  <\/jats:p>","DOI":"10.1111\/omi.12151","type":"journal-article","created":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T06:22:59Z","timestamp":1450678979000},"page":"35-47","source":"Crossref","is-referenced-by-count":33,"title":["The\n                    <i>Porphyromonas gingivalis<\/i>\n                    hemagglutinins HagB and HagC are major mediators of adhesion and biofilm formation"],"prefix":"10.1111","volume":"32","author":[{"given":"E.","family":"Connolly","sequence":"first","affiliation":[{"name":"Division of Oral Biosciences Dublin Dental University Hospital, School of Dental Science, Trinity College Dublin, University of Dublin Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Millhouse","sequence":"additional","affiliation":[{"name":"Infection and Immunity Research Group, Glasgow Dental School, School of Medicine College of Medical, Veterinary and Life Sciences, School of Dental Science, University of Glasgow Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Doyle","sequence":"additional","affiliation":[{"name":"Division of Oral Biosciences Dublin Dental University Hospital, School of Dental Science, Trinity College Dublin, University of Dublin Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Culshaw","sequence":"additional","affiliation":[{"name":"Infection and Immunity Research Group, Glasgow Dental School, School of Medicine College of Medical, Veterinary and Life Sciences, School of Dental Science, University of Glasgow Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Ramage","sequence":"additional","affiliation":[{"name":"Infection and Immunity Research Group, Glasgow Dental School, School of Medicine College of Medical, Veterinary 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