{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:04:54Z","timestamp":1789682694399,"version":"4.0.1"},"reference-count":76,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,5,22]],"date-time":"2017-05-22T00:00:00Z","timestamp":1495411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P26836\u2010B22"],"award-info":[{"award-number":["P26836\u2010B22"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P24317\u2010B22"],"award-info":[{"award-number":["P24317\u2010B22"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005221","name":"Biological Interactions Doctoral Programme","doi-asserted-by":"publisher","award":["W1224"],"award-info":[{"award-number":["W1224"]}],"id":[{"id":"10.13039\/501100005221","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>As a member of subgingival multispecies biofilms, <jats:italic>Tannerella forsythia<\/jats:italic> is commonly associated with periodontitis. The bacterium has a characteristic cell surface (S\u2010) layer modified with a unique <jats:italic>O<\/jats:italic>\u2010glycan. Both the S\u2010layer and the <jats:italic>O<\/jats:italic>\u2010glycan were analyzed in this study for their role in biofilm formation by employing an <jats:italic>in vitro<\/jats:italic> multispecies biofilm model mimicking the situation in the oral cavity. Different <jats:italic>T.\u00a0forsythia<\/jats:italic> strains and mutants with characterized defects in cell surface composition were incorporated into the model, together with nine species of select oral bacteria. The influence of the <jats:italic>T.\u00a0forsythia<\/jats:italic> S\u2010layer and attached glycan on the bacterial composition of the biofilms was analyzed quantitatively using colony\u2010forming unit counts and quantitative real\u2010time polymerase chain reaction, as well as qualitatively by fluorescence <jats:italic>in situ<\/jats:italic> hybridization and confocal laser scanning microscopy. This revealed that changes in the <jats:italic>T.\u00a0forsythia<\/jats:italic> cell surface did not affect the quantitative composition of the multispecies consortium, with the exception of <jats:italic>Campylobacter rectus<\/jats:italic> cell numbers. The localization of <jats:italic>T.\u00a0forsythia<\/jats:italic> within the bacterial agglomeration varied depending on changes in the S\u2010layer glycan, and this also affected its aggregation with <jats:italic>Porphyromonas gingivalis<\/jats:italic>. This suggests a selective role for the glycosylated <jats:italic>T.\u00a0forsythia<\/jats:italic> S\u2010layer in the positioning of this species within the biofilm, its co\u2010localization with <jats:italic>P.\u00a0gingivalis<\/jats:italic>, and the prevalence of <jats:italic>C.\u00a0rectus<\/jats:italic>. These findings might translate into a potential role of <jats:italic>T.\u00a0forsythia<\/jats:italic> cell surface structures in the virulence of this species when interacting with host tissues and the immune system, from within or beyond the biofilm.<\/jats:p>","DOI":"10.1111\/omi.12182","type":"journal-article","created":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T04:40:25Z","timestamp":1491453625000},"page":"404-418","source":"Crossref","is-referenced-by-count":34,"title":["Behavior of two <i>Tannerella forsythia<\/i> strains and their cell surface mutants in multispecies oral biofilms"],"prefix":"10.1111","volume":"32","author":[{"given":"Susanne","family":"Bloch","sequence":"first","affiliation":[{"name":"Department of NanoBiotechnology <i>NanoGlycobiology<\/i> unit Universit\u00e4t f\u00fcr Bodenkultur Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Thurnheer","sequence":"additional","affiliation":[{"name":"Division of Oral Microbiology and Immunology Institute of Oral Biology Center of Dental Medicine University of Z\u00fcrich  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukitaka","family":"Murakami","sequence":"additional","affiliation":[{"name":"Department of Oral Microbiology Asahi University School of Dentistry Mizuho  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios N.","family":"Belibasakis","sequence":"additional","affiliation":[{"name":"Division of Cariology and Endodontics Department of Dental Medicine Karolinska Institute  Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1613-7258","authenticated-orcid":false,"given":"Christina","family":"Sch\u00e4ffer","sequence":"additional","affiliation":[{"name":"Department of NanoBiotechnology <i>NanoGlycobiology<\/i> unit Universit\u00e4t f\u00fcr Bodenkultur Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00790.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000077756"},{"key":"e_1_2_7_4_1","first-page":"3","article-title":"Risk factors for periodontitis","volume":"7","author":"Van Dyke TE","year":"2005","journal-title":"J Int Acad Periodontol"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2012.00663.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2013.09.001"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.4.1244-1263.1998"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2012.02579.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.01364.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)37046-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.5121-5130.2001"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-014-0258-7"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01038-08"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/CBI20090290"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510385242"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.11.3130-3134.2002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.2.347-351.1990"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2004.00377.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20213"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.3.1954-1957.2006"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2462-2467.2006"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.03.009"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2009.00332.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26535-0"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-012-0792-3"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.284893"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12062"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.080192-0"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.2.155-166.2002"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnsc.2008.04.001"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.38.2.637-644.1982"},{"key":"e_1_2_7_33_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1128\/iai.35.1.157-165.1982","article-title":"Positive coooperativity in the binding of Streptococcus sanguis to hydroxylapatite","volume":"35","author":"Nesbitt WE","year":"1982","journal-title":"Infect Immun"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01544-06"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00029-11"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05100.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2007.09.006"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104529200"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr000407m"},{"key":"e_1_2_7_40_1","first-page":"342","article-title":"Tannerella forsythia strains display different cell\u2010surface nonulosonic acids: Biosynthetic pathway characterization and first insight into biological implications","volume":"27","author":"Friedrich V","year":"2017","journal-title":"Glycobiology"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/2.1.25"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.29275-0"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510384622"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2007.01.003"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-280"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-227"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083090"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12034"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2013.130167"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12108"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00660-15"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01286-16"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00139-13"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12104"},{"key":"e_1_2_7_55_1","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1128\/iai.42.2.459-470.1983","article-title":"Antigenic heterogeneity of Bacteroides intermedius as recognized by monoclonal antibodies","volume":"42","author":"Gm\u00fcr R","year":"1983","journal-title":"Infect Immun"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2003.09.003"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1159\/000091065"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.027920-0"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.1.4.12778"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm400447z"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1557\/mrs.2011.65"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900448"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345000790021301"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1985.tb00936.x"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2003.01627.x"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-016-6254-x"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2011.191"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2014.11.017"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1990.tb00415.x"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.2002.7.1.43"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1996.tb00334.x"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00062-06"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2001.036004237.x"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2005.00774.x"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2016.46.1.2"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/010926-0"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009321"}],"container-title":["Molecular Oral Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fomi.12182","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/omi.12182","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T14:12:18Z","timestamp":1696255938000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/omi.12182"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,22]]},"references-count":76,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1111\/omi.12182"],"URL":"https:\/\/doi.org\/10.1111\/omi.12182","archive":["Portico"],"relation":{},"ISSN":["2041-1006","2041-1014"],"issn-type":[{"value":"2041-1006","type":"print"},{"value":"2041-1014","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,22]]}}}