{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T20:52:17Z","timestamp":1775163137636,"version":"3.50.1"},"reference-count":99,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T00:00:00Z","timestamp":1498435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003803","name":"University of Hong Kong","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Treponema denticola<\/jats:italic> and other species (phylotypes) of oral spirochetes are widely considered to play important etiological roles in periodontitis and other oral infections. The major surface protein (Msp) of <jats:italic>T.\u00a0denticola<\/jats:italic> is directly implicated in several pathological mechanisms. Here, we have analyzed <jats:italic>msp<\/jats:italic> sequence diversity across 68 strains of oral phylogroup 1 and 2 treponemes; including reference strains of <jats:italic>T.\u00a0denticola<\/jats:italic>,<jats:italic> Treponema putidum<\/jats:italic>,<jats:italic> Treponema medium<\/jats:italic>, \u2018<jats:italic>Treponema vincentii<\/jats:italic>\u2019, and \u2018<jats:italic>Treponema sinensis<\/jats:italic>\u2019. All encoded Msp proteins contained highly conserved, taxon\u2010specific signal peptides, and shared a predicted \u2018three\u2010domain\u2019 structure. A clone\u2010based strategy employing \u2018<jats:italic>msp<\/jats:italic>\u2010specific\u2019 polymerase chain reaction primers was used to analyze <jats:italic>msp<\/jats:italic> gene sequence diversity present in subgingival plaque samples collected from a group of individuals with chronic periodontitis (n=10), vs periodontitis\u2010free controls (n=10). We obtained 626 clinical <jats:italic>msp<\/jats:italic> gene sequences, which were assigned to 21 distinct \u2018clinical <jats:italic>msp<\/jats:italic> genotypes\u2019 (95% sequence identity cut\u2010off). The most frequently detected clinical <jats:italic>msp<\/jats:italic> genotype corresponded to <jats:italic>T.\u00a0denticola <\/jats:italic><jats:styled-content style=\"fixed-case\">ATCC<\/jats:styled-content> 35405<jats:sup>T<\/jats:sup>, but this was not correlated to disease status. <jats:sc>UniFrac<\/jats:sc> and <jats:sc>libshuff<\/jats:sc> analysis revealed that individuals with periodontitis and periodontitis\u2010free controls harbored significantly different communities of treponeme clinical <jats:italic>msp<\/jats:italic> genotypes (<jats:italic>P<\/jats:italic>&lt;.001). Patients with periodontitis had higher levels of clinical <jats:italic>msp<\/jats:italic> genotype diversity than periodontitis\u2010free controls (Mann\u2010Whitney <jats:italic>U<\/jats:italic>\u2010test, <jats:italic>P<\/jats:italic>&lt;.05). The relative proportions of \u2018<jats:italic>T.\u00a0vincentii<\/jats:italic>\u2019 clinical <jats:italic>msp<\/jats:italic> genotypes were significantly higher in the control group than in the periodontitis group (<jats:italic>P<\/jats:italic>=.018). In conclusion, our data clearly show that both healthy and diseased individuals commonly harbor a wide diversity of <jats:italic>Treponema<\/jats:italic> clinical <jats:italic>msp<\/jats:italic> genotypes within their subgingival niches.<\/jats:p>","DOI":"10.1111\/omi.12185","type":"journal-article","created":{"date-parts":[[2017,4,28]],"date-time":"2017-04-28T17:25:29Z","timestamp":1493400329000},"page":"455-474","source":"Crossref","is-referenced-by-count":12,"title":["Oral treponeme major surface protein: Sequence diversity and distributions within periodontal niches"],"prefix":"10.1111","volume":"32","author":[{"given":"M.","family":"You","sequence":"first","affiliation":[{"name":"Department of Oral Radiology and State Key Laboratory of Oral Diseases West China College of Stomatology Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Chan","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry The 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