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Attachment to a biotic surface is a critical first step in the <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic> infection process for which exopolysaccharides have been shown to be essential. In addition, the <jats:italic>pga<\/jats:italic> operon, containing genes encoding for biosynthetic proteins for poly\u2010<jats:italic>N<\/jats:italic>\u2010acetyl glucosamine (<jats:styled-content style=\"fixed-case\">PNAG<\/jats:styled-content>), plays a key role in <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic> virulence, as a mutant strain lacking the <jats:italic>pga<\/jats:italic> operon induces significantly less bone resorption. Among the genes in the <jats:italic>pga<\/jats:italic> operon, <jats:italic>pgaB<\/jats:italic> codes for a de\u2010<jats:italic>N<\/jats:italic>\u2010acetylase that is responsible for the deacetylation of the <jats:styled-content style=\"fixed-case\">PNAG<\/jats:styled-content> exopolysaccharide. Here we report the role of PgaB in regulation of virulence genes using a markerless, scarless deletion mutant targeting the coding region of the N\u2010terminal catalytic domain of PgaB. The results demonstrate that the N\u2010terminal, catalytic domain of PgaB is crucial for exopolysaccharide export.<\/jats:p>","DOI":"10.1111\/omi.12188","type":"journal-article","created":{"date-parts":[[2017,5,26]],"date-time":"2017-05-26T11:22:42Z","timestamp":1495797762000},"page":"500-510","source":"Crossref","is-referenced-by-count":7,"title":["Role of de\u2010<i>N<\/i>\u2010acetylase PgaB from <i>Aggregatibacter actinomycetemcomitans<\/i> in exopolysaccharide export in biofilm mode of growth"],"prefix":"10.1111","volume":"32","author":[{"given":"M.","family":"Shanmugam","sequence":"first","affiliation":[{"name":"Department of Oral Biology Rutgers School of Dental Medicine  Newark NJ USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.O.","family":"Oyeniyi","sequence":"additional","affiliation":[{"name":"Department of Oral Biology Rutgers School of Dental Medicine  Newark NJ 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