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Therefore, inhibition of this organism in dental plaques has been one of the strategies for preventing and treating chronic periodontitis. We previously identified a Streptococcal ArcA derived Anti\u2010<jats:italic>P gingivalils<\/jats:italic> Peptide (SAPP) that in vitro, is capable of repressing the expression of several virulence genes in the organism. This leads to a significant reduction in <jats:italic>P gingivalis<\/jats:italic> virulence potential, including its ability to colonize on the surface of <jats:italic>Streptococcus gordonii<\/jats:italic>, to invade human oral epithelial cells, and to produce gingipains. In this study, we showed that SAPP had minimal cytotoxicity to human oral keratinocytes and gingival fibroblasts. We observed that SAPP directly bound to the cell surface of <jats:italic>P gingivalis<\/jats:italic>, and that alterations in the sequence at the N\u2010terminus of SAPP diminished its abilities to interact with <jats:italic>P gingivalis<\/jats:italic> cells and repressed the expression of virulence genes. Most strikingly, we demonstrated using an ex\u2010vivo assay that besides its inhibitory activity against <jats:italic>P gingivalis<\/jats:italic> colonization, SAPP could also reduce the levels of several other oral Gram\u2010negative bacteria strongly associated with periodontitis in multispecies biofilms. Our results provide a platform for the development of SAPP\u2010targeted therapeutics against chronic periodontitis.<\/jats:p>","DOI":"10.1111\/omi.12246","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T03:19:31Z","timestamp":1539055171000},"page":"430-439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Characterization and development of SAPP as a specific peptidic inhibitor that targets <i>Porphyromonas gingivalis<\/i>"],"prefix":"10.1111","volume":"33","author":[{"given":"Meng\u2010Hsuan","family":"Ho","sequence":"first","affiliation":[{"name":"Department of Oral Biology Meharry Medical College Nashville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3147-5039","authenticated-orcid":false,"given":"Richard J.","family":"Lamont","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology and 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