{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T10:55:00Z","timestamp":1777373700597,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T00:00:00Z","timestamp":1582243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1123866"],"award-info":[{"award-number":["1123866"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004799","name":"Australian Dental Research Foundation","doi-asserted-by":"publisher","award":["349\u20102018"],"award-info":[{"award-number":["349\u20102018"]}],"id":[{"id":"10.13039\/501100004799","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    is an anaerobic, gram\u2010negative human oral pathogen highly associated with chronic periodontitis.\n                    <jats:italic>P.\u00a0gingivalis<\/jats:italic>\n                    utilizes the type IX secretion system (T9SS) to transport many of its virulence factors including the gingipains to the cell surface. The T9SS is comprised of at least 16 proteins and the involvement of these 16 proteins in the T9SS has been verified by creating gene deletion mutants in\n                    <jats:italic>P.\u00a0gingivalis<\/jats:italic>\n                    . These T9SS mutants are regularly utilized to understand how these proteins function together to allow the secretion of the T9SS substrates. We performed label\u2010free quantitative proteomic analysis on the T9SS protein mutants in\n                    <jats:italic>P.\u00a0gingivalis<\/jats:italic>\n                    to understand the relative abundance of each T9SS component in different mutants. The T9SS components were reduced in abundance in the\n                    <jats:italic>porK<\/jats:italic>\n                    ,\n                    <jats:italic>porL<\/jats:italic>\n                    ,\n                    <jats:italic>porM<\/jats:italic>\n                    ,\n                    <jats:italic>porN<\/jats:italic>\n                    ,\n                    <jats:italic>sov<\/jats:italic>\n                    and\n                    <jats:italic>porT<\/jats:italic>\n                    mutants, whereas they were increased in the\n                    <jats:italic>porE<\/jats:italic>\n                    ,\n                    <jats:italic>porU<\/jats:italic>\n                    ,\n                    <jats:italic>porV<\/jats:italic>\n                    ,\n                    <jats:italic>porZ<\/jats:italic>\n                    and\n                    <jats:italic>porQ<\/jats:italic>\n                    mutants. Sov and PorW appear to be the lowest in abundance and PorV the highest amongst all the T9SS components in\n                    <jats:italic>P.\u00a0gingivalis<\/jats:italic>\n                    wild\u2010type strain. These results are consistent with the proposed role of Sov as the translocation pore in the outer membrane and PorV as the shuttle protein that transports the T9SS substrates between sub\u2010complexes. Together, the label\u2010free quantitative proteomics analyses showed that different T9SS mutants have vastly different abundances of the T9SS components. This knowledge will greatly assist in interpreting the phenotype of the T9SS mutants as well as selecting the right mutant for exploring the role of an individual component.\n                  <\/jats:p>","DOI":"10.1111\/omi.12283","type":"journal-article","created":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T09:05:15Z","timestamp":1581325515000},"page":"78-84","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Quantitative proteomic analysis of the type IX secretion system mutants in\n                    <i>Porphyromonas gingivalis<\/i>"],"prefix":"10.1111","volume":"35","author":[{"given":"Dhana G.","family":"Gorasia","sequence":"first","affiliation":[{"name":"Oral Health Cooperative Research Centre Melbourne Dental School Bio21 Institute The University of Melbourne Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle D.","family":"Glew","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre Melbourne Dental School Bio21 Institute The University of Melbourne Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul D.","family":"Veith","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre Melbourne Dental School Bio21 Institute The University of Melbourne Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6618-4856","authenticated-orcid":false,"given":"Eric C.","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre Melbourne Dental School Bio21 Institute The University of Melbourne Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07530.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.031591"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034512453589"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aau3333"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-09412-w"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.369223"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005152"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005820"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2873"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201344202"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0164313"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389203033487027"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep23288"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep37708"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00215"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0693-y"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02784-7"},{"issue":"14","key":"e_1_2_6_19_1","first-page":"S8","article-title":"Periodontal systemic associations: Review of the evidence","volume":"40","author":"Linden G. 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