{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:54:12Z","timestamp":1772160852102,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T00:00:00Z","timestamp":1590710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102018R1A5A2024418"],"award-info":[{"award-number":["NRF\u20102018R1A5A2024418"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102019R1A2C2007041"],"award-info":[{"award-number":["NRF\u20102019R1A2C2007041"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HI17C1377"],"award-info":[{"award-number":["HI17C1377"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Periodontitis is a chronic inflammatory disease of the gum caused by infection with multispecies oral bacteria. Since the periodontopathic bacteria,\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    together with\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    are frequently detected in patients with a severe form of periodontitis, interactions between their virulence factors might play an important role in progression of the disease.\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    and\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    possess lipopolysaccharide (Pg.LPS) and lipoteichoic acid (Ef.LTA), respectively, as the major virulence factors inducing inflammatory responses. However, the combinatorial effect of these virulence factors on chemokine expression was poorly understood. Here, we examined the interaction between Ef.LTA and Pg.LPS on IL\u20108 induction in human periodontal ligament (PDL) cells. Pg.LPS, but not Ef.LTA, induced IL\u20108 expression at both mRNA and protein levels, which was suppressed in the presence of Ef.LTA. Although Ef.LTA and Pg.LPS could stimulate Toll\u2010like receptor 2 (TLR2), Ef.LTA did not interfere with Pg.LPS induced\u2010TLR2 activation. However, Ef.LTA decreased Pg.LPS\u2010induced phosphorylation of ERK, JNK, and p38 kinase. Furthermore, Ef.LTA suppressed Pg.LPS\u2010induced IL\u20108 promoter activity as well as AP\u20101, NF\u2010IL6 and NF\u2010\u03baB transcription factors, which are indispensable for IL\u20108 expression. Interestingly, Ef.LTA enhanced only IL\u20101 receptor\u2010associated kinase\u2010M (IRAK\u2010M) expression among the tested negative regulators of TLR intracellular signaling cascades in the presence of Pg.LPS. In addition, silencing IRAK\u2010M restored the decreased IL\u20108 expression by Ef.LTA in the presence of Pg.LPS. Collectively, these results suggest that Ef.LTA inhibits Pg.LPS\u2010induced IL\u20108 expression in human PDL cells via inducing the expression of a negative regulator of TLR signaling cascades, IRAK\u2010M.\n                  <\/jats:p>","DOI":"10.1111\/omi.12287","type":"journal-article","created":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T14:04:49Z","timestamp":1587391489000},"page":"146-157","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Lipoteichoic acid of\n                    <i>Enterococcus faecalis<\/i>\n                    interferes with\n                    <i>Porphyromonas gingivalis<\/i>\n                    lipopolysaccharide signaling via IRAK\u2010M upregulation in human periodontal ligament cells"],"prefix":"10.1111","volume":"35","author":[{"given":"Jintaek","family":"Im","sequence":"first","affiliation":[{"name":"Department of Oral Microbiology and Immunology DRI, and BK21 Plus Program School of Dentistry Seoul National University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung Eun","family":"Baik","sequence":"additional","affiliation":[{"name":"Department of Oral Microbiology and Immunology DRI, and BK21 Plus Program School of Dentistry Seoul National University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongwook","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Oral Microbiology and Immunology DRI, and BK21 Plus Program School of Dentistry Seoul National University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kee\u2010Yeon","family":"Kum","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry DRI School of Dentistry Seoul National University Seoul Republic of Korea"},{"name":"National Dental Care Center for Persons with Special Needs Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheol\u2010Heui","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences Seoul National University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ok\u2010Jin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Oral Microbiology and Immunology DRI, and BK21 Plus Program School of Dentistry Seoul National University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9418-9278","authenticated-orcid":false,"given":"Seung Hyun","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Oral Microbiology and Immunology DRI, and BK21 Plus Program School of Dentistry Seoul National University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2017.08.004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2010.11.007"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21287"},{"key":"e_1_2_7_5_1","first-page":"1","article-title":"Mammalian MAP kinase modules: How to transduce specific signals","volume":"32","author":"Brunet A.","year":"1997","journal-title":"Essays in Biochemistry"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6799-6807.2003"},{"key":"e_1_2_7_7_1","first-page":"2015, 549691","article-title":"PPAR\u03b3 and the innate immune system mediate the resolution of inflammation","author":"Croasdell A.","year":"2015","journal-title":"PPAR Research"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8091339"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00360.2002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1753425907088244"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-014-1249-7"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e31822a22f7"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000006932"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.5487\/TR.2018.34.1.055"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00730.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2009.01.013"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-73"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1477-1482.2001"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.72.5.847"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2013.10.004"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00053"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00045"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxv016"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.09.004"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2015.01.022"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2013.86"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00262"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.08.015"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401850"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2017.09.009"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00827-9"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.9.1329"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1630"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2011.110406"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100702108"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.4.2257"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7062"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16190-y"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02234-4"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.2741\/2353"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2015.05.008"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1012522"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.120050"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2008.10.014"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.5114\/pwki.2013.35445"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066914"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2005.10.049"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2010.100004"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0113003"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis290"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.46620"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2017.11.005"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2014.05.011"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2017.02.011"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.4167\/jbv.2015.45.4.364"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1017\/erm.2013.8"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-017-1052-x"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2013.2"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1273082"}],"container-title":["Molecular Oral Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fomi.12287","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/omi.12287","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/omi.12287","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/omi.12287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T16:23:19Z","timestamp":1693930999000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/omi.12287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,29]]},"references-count":58,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["10.1111\/omi.12287"],"URL":"https:\/\/doi.org\/10.1111\/omi.12287","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/OMI.12287\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/OMI.12287\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/OMI.12287\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/OMI.12287\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/OMI.12287\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/OMI.12287\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/OMI.12287\/v2\/review2","asserted-by":"object"}]},"ISSN":["2041-1006","2041-1014"],"issn-type":[{"value":"2041-1006","type":"print"},{"value":"2041-1014","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,29]]},"assertion":[{"value":"2019-11-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}