{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T21:33:53Z","timestamp":1780608833968,"version":"3.54.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T00:00:00Z","timestamp":1607472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Treponema denticola<\/jats:italic> is a proteolytic anaerobic spirochete and key contributor to periodontal disease of microbial etiology. As periodontal disease develops and progresses, <jats:italic>T. denticola<\/jats:italic> thrives in the hostile environment of the subgingival crevice by exploiting the negative regulatory activity of the complement protein, factor H (FH). FH bound to the cell surface receptor, FhbB (FH binding protein B), is competent to serve as a cofactor for the Factor I mediated\u2010cleavage of the opsonin C3b. However, bound FH is ultimately cleaved by the <jats:italic>T. denticola<\/jats:italic> protease, dentilisin. As the <jats:italic>T. denticola<\/jats:italic> population expands, the rate of FH cleavage may exceed its rate of replenishment leading to local FH depletion and immune dysregulation culminating in tissue and ligament destruction and tooth loss. The goal of this study was to develop a <jats:italic>T. denticola<\/jats:italic> FhbB based\u2010vaccine antigen that can block FH binding and cleavage and kill cells via antibody\u2010mediated bactericidal activity. Tetra (FhbB\u2010ch4) and pentavalent <jats:italic>fhbB<\/jats:italic> (FhbB\u2010ch5) chimerics were engineered to have attenuated FH binding ability. The chimerics were immunogenic and elicited high\u2010titer bactericidal and agglutinating antibody. Anti\u2010Fhb\u2010ch4 antisera blocked FH binding and cleavage by the <jats:italic>T. denticola<\/jats:italic> protease, dentilisin, in a dose dependent manner. Precedent for the use of FH binding proteins comes from the successful development of two FDA approved vaccines for type B <jats:italic>Neiserria meningitidis<\/jats:italic>. This study is the first to extend this approach to the development of a preventive or therapeutic vaccine (or monoclonal Ab) for periodontal disease.<\/jats:p>","DOI":"10.1111\/omi.12325","type":"journal-article","created":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T08:44:29Z","timestamp":1605948269000},"page":"50-57","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development of an FhbB based chimeric vaccinogen that elicits antibodies that block Factor H binding and cleavage by the periopathogen Treponema denticola"],"prefix":"10.1111","volume":"36","author":[{"given":"Nathaniel S.","family":"O'Bier","sequence":"first","affiliation":[{"name":"Department Microbiology and Immunology Virginia Commonwealth University Medical Center Richmond VA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhara T.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department Microbiology and Immunology Virginia Commonwealth University Medical Center Richmond VA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Lee D.","family":"Oliver","sequence":"additional","affiliation":[{"name":"Department Microbiology and Immunology Virginia Commonwealth University Medical Center Richmond VA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department Microbiology and Immunology Virginia Commonwealth University Medical Center Richmond VA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6035-7306","authenticated-orcid":false,"given":"Richard T.","family":"Marconi","sequence":"additional","affiliation":[{"name":"Department Microbiology and Immunology Virginia Commonwealth University Medical Center Richmond VA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01231-19"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003470"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002688"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40121-020-00319-0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40121-020-00322-5"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2020.07.034"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12077"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2003.08.001"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12203"},{"key":"e_1_2_6_11_1","first-page":"357","volume-title":"Pathogenic Treponema: Molecular and cellular biology","author":"Ellen R. 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