{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T02:11:36Z","timestamp":1782267096275,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T00:00:00Z","timestamp":1665446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100015499","name":"Jeonbuk National University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100015499","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Dental caries (tooth\u2010decay) is caused by biofilms harboring polymicrobial communities on teeth that leads to the onset of localized areas of enamel demineralization.\n                    <jats:italic>Streptococcus mutans<\/jats:italic>\n                    has been clinically associated with severe caries in childhood. Although commensal bacteria can combat\n                    <jats:italic>S. mutans<\/jats:italic>\n                    using self\u2010generated antimicrobials such as hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ), constant sugar\u2010rich diet consumption disrupts microbial homeostasis shifting toward cariogenic community. Recently,\n                    <jats:italic>Streptococcus oralis<\/jats:italic>\n                    subsp.\n                    <jats:italic>tigurinus<\/jats:italic>\n                    strain J22, an oral isolate, was identified as a uniquely potent H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    producer. Here, we assess whether a high H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010producing commensal streptococcus can modulate the spatial organization and virulence of\n                    <jats:italic>S. mutans<\/jats:italic>\n                    within biofilms. Using an experimental biofilm model, we find that the presence of\n                    <jats:italic>S. oralis<\/jats:italic>\n                    J22 can effectively inhibit the clustering, accumulation, and spatial organization of\n                    <jats:italic>S. mutans<\/jats:italic>\n                    on ex vivo human tooth surface, resulting in significant reduction of enamel demineralization. Notably, the generation of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    via pyruvate oxidase (SpxB) from\n                    <jats:italic>S. oralis<\/jats:italic>\n                    J22 is not repressed by sugars (a common repressor in other mitis group streptococci), resulting in enhanced inhibition of\n                    <jats:italic>S. mutans<\/jats:italic>\n                    growth (vs.\n                    <jats:italic>Streptococcus gordonii<\/jats:italic>\n                    ). We further investigate its impact on biofilm virulence using an in vivo rodent caries model under sugar\u2010rich diet. Coinfection of\n                    <jats:italic>S. mutans<\/jats:italic>\n                    with\n                    <jats:italic>S. oralis<\/jats:italic>\n                    results in reduced caries development compared to either species infected alone, whereas coinfection with\n                    <jats:italic>S. gordonii<\/jats:italic>\n                    has negligible effects, suggesting that the presence of an efficient, high H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010producer can disrupt\n                    <jats:italic>S. mutans<\/jats:italic>\n                    virulence. This work demonstrates that oral isolates with unusual high H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    production may be capable of modulating biofilm cariogenicity in vivo. The findings also highlight the importance of bacterial antagonistic interactions within polymicrobial communities in health and in disease\u2010causing state.\n                  <\/jats:p>","DOI":"10.1111\/omi.12394","type":"journal-article","created":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T05:28:49Z","timestamp":1664170129000},"page":"244-255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Antagonistic interactions by a high H\n                    <sub>2<\/sub>\n                    O\n                    <sub>2<\/sub>\n                    \u2010producing commensal streptococcus modulate caries development by\n                    <i>Streptococcus mutans<\/i>"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6185-9463","authenticated-orcid":false,"given":"Dongyeop","family":"Kim","sequence":"first","affiliation":[{"name":"Biofilm Research Laboratory Department of Orthodontics School of Dental Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"},{"name":"Department of Preventive Dentistry School of Dentistry and Institute of Oral Bioscience Jeonbuk National University  Jeonju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuro","family":"Ito","sequence":"additional","affiliation":[{"name":"Biofilm Research Laboratory Department of Orthodontics School of Dental Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"},{"name":"Department of Pediatric Dentistry School of Dentistry at Matsudo Nihon University  Matsudo Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson","family":"Hara","sequence":"additional","affiliation":[{"name":"Department of Cariology, Operative Dentistry and Dental Public Health, Oral Health Research Institute Indiana University School of Dentistry  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Li","sequence":"additional","affiliation":[{"name":"Biofilm Research Laboratory Department of Orthodontics School of Dental Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Kreth","sequence":"additional","affiliation":[{"name":"Departments of Restorative Dentistry and Molecular Microbiology &amp; Immunology Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun","family":"Koo","sequence":"additional","affiliation":[{"name":"Biofilm Research Laboratory Department of Orthodontics School of Dental Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"},{"name":"Divisions of Pediatric Dentistry &amp; Community Oral Health School of Dental Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"},{"name":"Center for Innovation &amp; Precision Dentistry School of Dental Medicine School of Engineering and Applied Sciences University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/BI00453A032"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2017.09.008"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01697\u201017"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2017.1307079"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02490\u201020"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00542\u201010"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1409644111"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12152"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00021\u201016"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02320\u201010"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001433"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515609554"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396\u2010020\u201000789\u20107"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034520909313"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1919099117"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396\u2010018\u20100113\u20101"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41332"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.2041\u20101014.2012.00647.X"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2381"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.21.7193\u20107203.2005"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010018\u20100089\u2010x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201005342\u2010x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1522149113"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413\u2010019\u20100061\u2010z"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.2041\u20101014.2011.00634.X"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.12953"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/OMI.12348"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00203\u2010013\u20100896\u20104"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.30"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12231"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00619\u201017"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396\u2010020\u20100592\u20108"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348\u20100421.2007.tb03971.x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034512442894"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12216"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034518790941"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002623"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00309\u201011"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/717843"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086685"}],"container-title":["Molecular 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