{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:36:12Z","timestamp":1791945372442,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T00:00:00Z","timestamp":1674864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The Msp protein complex and the serine protease dentilisin are the best\u2010characterized virulence factors in\n                    <jats:italic>Treponema denticola<\/jats:italic>\n                    , the major etiological agent of chronic periodontitis. In addition to these outer sheath factors, the cysteine protease dentipain contributes to pathogenicity, but its secretion, processing, cellular localization, and role in\n                    <jats:italic>T. denticola<\/jats:italic>\n                    virulence are not fully understood. In this study, we found that full\u2010sized dentipain (74\u2010kDa) and the 52\u2010kDa truncated form of the enzyme are located, respectively, in the outer sheath derived from\n                    <jats:italic>T. denticola<\/jats:italic>\n                    dentilisin\u2010 and the Msp\u2010deficient mutants. Furthermore, dentipain was barely detected in the wild\u2010type strain. These results suggest that dentilisin and Msp, the major outer sheath proteins, are involved in the secretion and maturation of dentipain. Inactivation of the dentipain gene slowed the growth of\n                    <jats:italic>T. denticola<\/jats:italic>\n                    , and the effect was more profound in serum\u2010free medium than in serum\u2010containing medium. Several genes, including those encoding transporters and methyl\u2010accepting chemotaxis proteins, were differentially expressed in the dentipain\u2010deficient mutant. Furthermore, the mutant strain was more hydrophobic than the wild\u2010type strain. Finally, the mutant showed less autoaggregation activity and adhesion to IgG in a serum\u2010free medium than the wild\u2010type strain. These findings suggest that dentipain contributes to the virulence of\n                    <jats:italic>T. denticola<\/jats:italic>\n                    by facilitating adhesion and acquisition of nutrients essential for colonization and proliferation in the gingival crevice under serum\u2010rich conditions.\n                  <\/jats:p>","DOI":"10.1111\/omi.12406","type":"journal-article","created":{"date-parts":[[2023,1,15]],"date-time":"2023-01-15T11:38:50Z","timestamp":1673782730000},"page":"212-223","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Localization and pathogenic role of the cysteine protease dentipain in\n                    <i>Treponema denticola<\/i>"],"prefix":"10.1111","volume":"38","author":[{"given":"Yuri","family":"Miyai\u2010Murai","sequence":"first","affiliation":[{"name":"Department of Crown and Bridge Prosthodontics Tokyo Dental College  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuko","family":"Okamoto\u2010Shibayama","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tokyo Dental College  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Crown and Bridge Prosthodontics Tokyo Dental College  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichiro","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tokyo Dental College  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eitoyo","family":"Kokubu","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tokyo Dental College  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Potempa","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Biochemistry Biophysics and Biotechnology Jagiellonian University  Krakow Poland"},{"name":"Department of Oral Immunity and Infectious Diseases University of Louisville School of Dentistry  Louisville Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6567-807X","authenticated-orcid":false,"given":"Kazuyuki","family":"Ishihara","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tokyo Dental College  Tokyo 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