{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:07:47Z","timestamp":1783008467317,"version":"3.54.5"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T00:00:00Z","timestamp":1694995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Several oral bacteria, including\n                    <jats:italic>Prevotella melaninogenica<\/jats:italic>\n                    (Pm), have aquaporin (AQP) proteins homologous to human AQP5, a major water channel protein targeted in Sjogren's syndrome. This study aimed to understand the antigenic characteristics that induce autoantibodies against an AQP5 \u201cE\u201d epitope (AQP5E) in a mouse model using C57BL\/6 mice. Immunization with a PmE\u2010L peptide derived from Pm AQP, which contains amino acid mismatches both at the B\u2010 and T\u2010cell epitopes, efficiently induced anti\u2010AQP5E autoantibodies accompanied by increased germinal center (GC) B and follicular helper T cells in the draining lymph nodes. However, PmE, a peptide lacking a T\u2010cell epitope, and AQP5E\u2010L, an AQP5\u2010derived self\u2010peptide, hardly induced either anti\u2010AQP5E autoantibodies or GC responses. Surprisingly, OTII\u2010AQP5E, a peptide that replaced the self T\u2010cell epitope of AQP5E\u2010L with an ovalbumin\u2010derived foreign T\u2010cell epitope, was not any better than AQP5E\u2010L in the induction of anti\u2010AQP5E autoantibodies and GC response, despite the substantial expansion of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells and production of anti\u2010OTII\u2010AQP5E antibodies. The complex of biotinylated PmE\u2010L peptide and highly immunogenic streptavidin (SA) induced a strong extrafollicular B\u2010cell response skewed toward the expansion of SA\u2010specific B cells. However, the expansion of AQP5E\u2010specific GC B cells was limited, resulting in the inefficient induction of anti\u2010AQP5E autoantibodies. Collectively, our results have demonstrated that anti\u2010AQP5E autoantibody production is only allowed when foreign B\u2010 and T\u2010cell epitopes drive a strong GC response of AQP5E\u2010specific B cells for affinity maturation. This study helps explain why cross\u2010reactive anti\u2010AQP5 autoantibodies are not produced during the immune response to Pm in most healthy people.\n                  <\/jats:p>","DOI":"10.1111\/omi.12430","type":"journal-article","created":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T04:10:15Z","timestamp":1695010215000},"page":"442-453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Requirements for anti\u2010aquaporin 5 autoantibody production in a mouse model"],"prefix":"10.1111","volume":"38","author":[{"given":"Sabin","family":"Acharya","sequence":"first","affiliation":[{"name":"Department of Immunology and Molecular Microbiology School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahreum","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Immunology and Molecular Microbiology School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunjin","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Immunology and Molecular Microbiology School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeong\u2010jin","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Immunology and Molecular Microbiology School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6496-5560","authenticated-orcid":false,"given":"Youngnim","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Immunology and Molecular Microbiology School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of 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