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However, persistent immune responses to the allograft affect chronic rejection, which induces OAT vasculopathy (OATV) and organ failure. <jats:italic>Porphyromonas gingivalis<\/jats:italic> can infiltrate remote organs via the bloodstream, thereby intensifying the severity of cardiovascular, respiratory, and neurodegenerative diseases and cancer. GroEL, a virulence factor of <jats:italic>P. gingivalis<\/jats:italic> promotes pro\u2010inflammatory cytokine production in host cells, which assumes to play a pivotal role in the pathogenesis of cardiovascular diseases. Although the aggravation of OATV is attributable to numerous factors, the role of GroEL remains ambiguous. Therefore, this study aimed to investigate the impact of GroEL on OATV. Aortic grafts extracted from PVG\/Seac rats were transplanted into ACI\/NKyo rats and in vitro human endothelial progenitor cell (EPC) and coronary artery endothelial cell (HCAEC) models. The experimental findings revealed that GroEL exacerbates OATV in ACI\/NKyo rats by affecting EPC and smooth muscle progenitor cell (SMPC) function and enabling the anomalous accumulation of collagen. In vitro, GroEL spurs endothelial\u2010mesenchymal transition in EPCs, reduces HCAEC tube formation and barrier function by downregulating junction proteins, accelerates HCAEC aging by lowering mitochondrial membrane potential and respiratory function, and impedes HCAEC migration by modulating cytoskeleton\u2010associated molecules. This study suggests that <jats:italic>P. gingivalis<\/jats:italic> GroEL could potentially augment OATV by impacting vascular progenitor and endothelial cell functions.<\/jats:p>","DOI":"10.1111\/omi.12453","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T06:42:06Z","timestamp":1707288126000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>Porphyromonas gingivalis<\/i> GroEL exacerbates orthotopic allograft transplantation vasculopathy via impairment of endothelial cell function"],"prefix":"10.1111","author":[{"given":"Chien\u2010Sung","family":"Tsai","sequence":"first","affiliation":[{"name":"Division of Cardiovascular Surgery Tri\u2010Service General Hospital, National Defense Medical Center  Taipei Taiwan"},{"name":"Department and Graduate Institute of Pharmacology National Defense Medical Center  Taipei Taiwan"},{"name":"Taipei Heart Institute Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Yao","family":"Huang","sequence":"additional","affiliation":[{"name":"Taipei Heart Institute Taipei Medical University  Taipei Taiwan"},{"name":"Division of Cardiology and Cardiovascular Research Center Taipei Medical University Hospital  Taipei Taiwan"},{"name":"Division of Cardiology Department of Internal Medicine School of Medicine, College of Medicine, Taipei Medical University  Taipei Taiwan"},{"name":"Department of Biomedical Sciences and Engineering National Central University  Taoyuan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Ting","family":"Tsai","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Surgery Tri\u2010Service General Hospital, National Defense Medical Center  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Ming","family":"Shih","sequence":"additional","affiliation":[{"name":"Taipei Heart Institute Taipei Medical University  Taipei Taiwan"},{"name":"Division of Cardiology and Cardiovascular Research Center Taipei Medical University Hospital  Taipei Taiwan"},{"name":"Division of Cardiology Department of Internal Medicine School of Medicine, College of Medicine, Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ze\u2010Hao","family":"Lai","sequence":"additional","affiliation":[{"name":"Institute of Oral Biology National Yang Ming Chiao Tung University (Taipei Campus)  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen\u2010Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Science College of Medicine University of Arizona  Phoenix Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Wen","family":"Lin","sequence":"additional","affiliation":[{"name":"Taipei Heart Institute Taipei Medical University  Taipei Taiwan"},{"name":"Institute of Oral Biology National Yang Ming Chiao Tung University (Taipei Campus)  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4939-2899","authenticated-orcid":false,"given":"Feng\u2010Yen","family":"Lin","sequence":"additional","affiliation":[{"name":"Taipei Heart Institute Taipei Medical University  Taipei Taiwan"},{"name":"Division of Cardiology and Cardiovascular Research Center Taipei Medical University Hospital  Taipei Taiwan"},{"name":"Division of Cardiology Department of Internal Medicine School of Medicine, College of Medicine, Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,4]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26185653"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.11.1463"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399\u2010302X.2006.00286.x"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1053\u20102498(03)00222\u20105"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20105223(00)70004\u20103"},{"key":"e_1_2_12_7_1","doi-asserted-by":"crossref","unstructured":"Beck J. 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