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Hemagglutinin's functional domains include cleaved adhesin (CA), which acts as a hemagglutination and hemoglobin\u2010binding actor. Here, we confirmed that the HA and CA genes are immunogenic, and using adjuvant chemokine to target dendritic cells (DCs) enhanced protective autoimmunity against\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      \u2013induced periodontal disease.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      C57 mice were immunized prophylactically with pVAX1\u2010CA, pVAX1\u2010HA, pVAX1, and phosphate\u2010buffered saline (PBS) through intramuscular injection every 2 weeks for a total of three administrations before\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      \u2013induced periodontitis. The DCs were analyzed using flow cytometry and ribonucleic acid sequencing (RNA\u2010seq) transcriptomic assays following transfection with CA lentivirus. The efficacy of the co\u2010delivered molecular adjuvant CA DNA vaccine was evaluated in vivo using flow cytometry, immunofluorescence techniques, and micro\u2010computed tomography.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      After the immunization, both the pVAX1\u2010CA and pVAX1\u2010HA groups exhibited significantly elevated\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      \u2013specific IgG and IgG1, as well as a reduction in bone loss around periodontitis\u2010affected teeth, compared to the pVAX1 and PBS groups (\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      &lt;\u00a00.05). The expression of CA promoted the secretion of HLA, CD86, CD83, and DC\u2010specific intercellular adhesion molecule\u20103\u2010grabbing non\u2010integrin (DC\u2010SIGN) in DCs. Furthermore, the RNA\u2010seq analysis revealed a significant increase in the chemokine (C\u2013C motif) ligand 19 (\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      &lt;\u00a00.05). A notable elevation in the quantities of DCs co\u2010labeled with CD11c and major histocompatibility complex class II, along with an increase in interferon\u2010gamma (IFN\u2010\u03b3) cells, was observed in the inguinal lymph nodes of mice subjected to CCL19\u2010CA immunization. This outcome effectively illustrated the preservation of peri\u2010implant bone mass in rats afflicted with\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      \u2013induced peri\u2010implantitis (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The co\u2010administration of a CCL19\u2010conjugated CA DNA vaccine holds promise as an innovative and targeted immunization strategy against\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      \u2013induced periodontitis and peri\u2010implantitis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/omi.12465","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T11:50:24Z","timestamp":1714650624000},"page":"433-445","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A cleaved adhesin DNA vaccine targeting dendritic cell against\n                    <i>Porphyromonas gingivalis<\/i>\n                    \u2013induced periodontal disease"],"prefix":"10.1111","volume":"39","author":[{"given":"Xin","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Shandong Second Medical University  Weifang Shandong China"},{"name":"School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration &amp; Shandong Provincial Clinical Research Center for Oral Diseases  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng\u2010Yu","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Shandong Second Medical University  Weifang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke\u2010Feng","family":"Luan","sequence":"additional","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Shandong Second Medical University  Weifang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen\u2010Yu","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Shandong Second Medical University  Weifang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Ping","family":"Ren","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration &amp; Shandong Provincial Clinical Research Center for Oral Diseases  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue\u2010Hui","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Shandong Second Medical University  Weifang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Lan","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration &amp; Shandong Provincial Clinical Research Center for Oral Diseases  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4086"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600\u20100765.2003.02658.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(22)00524\u20104"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20032220"},{"issue":"3","key":"e_1_2_11_6_1","doi-asserted-by":"crossref","DOI":"10.1038\/cddis.2016.481","article-title":"Porphyromonas gingivalis gingipains cause defective macrophage migration towards apoptotic cells and inhibit phagocytosis of primary apoptotic neutrophils","volume":"8","author":"Castro S. 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