{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:41:16Z","timestamp":1793040076755,"version":"4.5.0"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DE030121"],"award-info":[{"award-number":["R01DE030121"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"Abstract<\/jats:title>\n \n Porphyromonas gingivalis<\/jats:italic>\n is a keystone pathogen in periodontitis, and\n Streptococcus sanguinis<\/jats:italic>\n is an abundant oral commensal bacterium associated with periodontal health. However, the interaction between\n P. gingivalis<\/jats:italic>\n and\n S. sanguinis<\/jats:italic>\n remains obscure. Here, we established a strategy for high\u2010throughput measurement of the cell number of\n P. gingivalis<\/jats:italic>\n in the coculture with\n S. sanguinis<\/jats:italic>\n by detecting the concentration of hydrogen sulfate. The interaction between\n P. gingivalis<\/jats:italic>\n and over 2000\n S. sanguinis<\/jats:italic>\n single\u2010gene mutants was characterized using this strategy, and several interaction\u2010associated genes in\n S. sanguinis<\/jats:italic>\n were determined by detecting more\n P. gingivalis<\/jats:italic>\n cells in the coculture with matched\n S. sanguinis<\/jats:italic>\n mutants. Three\n S. sanguinis<\/jats:italic>\n interaction\u2010associated genes were predicted to be responsible for cysteine metabolism, and the supplementation of exogenous L\u2010cysteine promoted the cell number of\n P. gingivalis<\/jats:italic>\n in the coculture with\n S. sanguinis<\/jats:italic>\n . Thus, exogenous L\u2010cysteine and the compromised cysteine metabolism in\n S. sanguinis<\/jats:italic>\n enhanced the growth of\n P. gingivalis<\/jats:italic>\n in the existence of\n S. sanguinis<\/jats:italic>\n . Additionally, the interaction between\n P. gingivalis<\/jats:italic>\n and other\n Streptococcus<\/jats:italic>\n spp. was examined, and\n S. pneumoniae<\/jats:italic>\n was the only streptococci that had no inhibition on the cell number of\n P. gingivalis<\/jats:italic>\n . In total, this study established a new strategy for high\u2010throughput screening of the interaction between\n Streptococcus<\/jats:italic>\n and\n P. gingivalis<\/jats:italic>\n and discovered a set of genes in\n S. sanguinis<\/jats:italic>\n that impacted the interaction. The influence of exogenous L\u2010cysteine on the interaction between\n P. gingivalis<\/jats:italic>\n and\n S. sanguinis<\/jats:italic>\n in the oral cavity needs further investigation.\n <\/jats:p>","DOI":"10.1111\/omi.12478","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T23:05:35Z","timestamp":1721948735000},"page":"461-467","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["High\u2010throughput characterization of the influence of\n Streptococcus sanguinis<\/i>\n genes on the interaction between\n Streptococcus sanguinis<\/i>\n and\n Porphyromonas gingivalis<\/i>"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2829-2925","authenticated-orcid":false,"given":"Bin","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Microbiology & Immunology School of Medicine Virginia Commonwealth University Richmond Virginia USA"},{"name":"Center for Microbiome Engineering and Data Analysis Virginia Commonwealth University Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vysakh","family":"Anandan","sequence":"additional","affiliation":[{"name":"The Philips Institute for Oral Health Research School of Dentistry Virginia Commonwealth University Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Bao","sequence":"additional","affiliation":[{"name":"The Philips Institute for Oral Health Research School of Dentistry Virginia Commonwealth University Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8368-801X","authenticated-orcid":false,"given":"Ping","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology School of Medicine Virginia Commonwealth University Richmond Virginia USA"},{"name":"Center for Microbiome Engineering and Data Analysis Virginia Commonwealth University Richmond Virginia USA"},{"name":"The Philips Institute for Oral Health Research School of Dentistry Virginia Commonwealth University Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.174"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11101173"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8MT00150B"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100765.1998.tb02326.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3791\/4356-v"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.191"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-023-01456-3"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12170"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2022.945523"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.956417"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.06.005"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1900101116"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000825"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky427"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00125"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.090012"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201002399\u20104"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2018-0043"},{"key":"e_1_2_9_20_1","first-page":"1","article-title":"Aggregatibacter actinomycetemcomitans mediates protection of Porphyromonas gingivalis from Streptococcus sanguinis hydrogen peroxide production in multi\u2010species biofilms","volume":"9","author":"Zhu B.","year":"2019","journal-title":"Scientific Reports"}],"container-title":["Molecular Oral Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/omi.12478","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T03:50:50Z","timestamp":1730692250000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/omi.12478"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,25]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/omi.12478"],"URL":"https:\/\/doi.org\/10.1111\/omi.12478","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/OMI.12478\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/OMI.12478\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/OMI.12478\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/OMI.12478\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/OMI.12478\/v2\/decision1","asserted-by":"object"}]},"ISSN":["2041-1006","2041-1014"],"issn-type":[{"value":"2041-1006","type":"print"},{"value":"2041-1014","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,25]]},"assertion":[{"value":"2023-10-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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