{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:50:03Z","timestamp":1747187403600,"version":"3.40.5"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T00:00:00Z","timestamp":1721952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Periodontitis is caused by a dysbiosis of oral bacteria resulting in alveolar bone destruction and teeth loss. The role of platelets in pathogenesis of periodontitis is a subject of research. The release of toxins from periodontitis\u2010associated bacteria may influence platelet function and contribute to the modulation of hemostatic or inflammatory responses. Therefore, we explored platelet function upon exposure to defined toxins: leukotoxin A from <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic> (LtxA), a synthetic version of the C14\u2010Tri\u2010LAN\u2010Gly peptide from <jats:italic>Fusobacterium nucleatum<\/jats:italic> (C14), and lipopolysaccharides from <jats:italic>Porphyromonas gingivalis<\/jats:italic> (LPS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Light transmission aggregometry was performed after the addition of toxins to platelet\u2010rich plasma in different doses. Flow cytometry was used to identify inhibitory effects of toxins by measuring phosphorylation of the vaso\u2010dilator\u2010stimulated phosphoprotein or to identify activating effects by the detection of CD62P expression. The release of chemokines derived from washed platelets was determined by immunoassays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Collagen\u2010induced threshold aggregation values were diminished upon incubation with LtxA and C14, accompanied with an increase of vaso\u2010dilator\u2010stimulated phosphoprotein (VASP) phosphorylation, indicating platelet inhibition. In contrast, LPS did not affect aggregation but slightly enhanced CD62P expression under co\u2010stimulation with low\u2010dose thrombin pointing to slight platelet activation. The three toxins did not relevantly influence the secretion of chemokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although weak, the investigated toxins differently influenced human platelet function. LtxA and C14 mediated inhibitory effects, whereas LPS contributed to a slight activation of platelets. Further analysis of specific cellular responses mediated by bacterial toxins may render novel targets and suggestions for the treatment of periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/omi.12480","type":"journal-article","created":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T10:44:19Z","timestamp":1721990659000},"page":"468-476","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of toxins from different periodontitis\u2010associated bacteria on human platelet function"],"prefix":"10.1111","volume":"39","author":[{"given":"Anna","family":"Kobsar","sequence":"first","affiliation":[{"name":"Institute of Transfusion Medicine and Haemotherapy University of Wuerzburg  Wurzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Wiebecke","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Haemotherapy University of Wuerzburg  Wurzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katja","family":"Weber","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Haemotherapy University of Wuerzburg  Wurzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Koessler","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Haemotherapy University of Wuerzburg  Wurzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Haemotherapy University of Wuerzburg  Wurzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Boeck","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Haemotherapy University of Wuerzburg  Wurzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Zeller\u2010Hahn","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Haemotherapy University of Wuerzburg  Wurzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8400-9552","authenticated-orcid":false,"given":"Juergen","family":"Koessler","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Haemotherapy University of Wuerzburg  Wurzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,26]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2010.10.023"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2015\u20102021.2006"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/636893"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112542"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12314"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2011.11.036"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400702"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH14\u201002\u20100146"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348\u20100421.12864"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2873"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12119"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12895"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010051x.1994.tb00737.x"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510363682"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.11.6094\u20106100.2000"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100039\u20101685495"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000155018.67835.1a"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.4.920\u2010929.1990"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12284"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2011.02.016"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109258(18)47421\u20102"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010051X.1986.tb00852.x"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.2015.1014470"},{"key":"e_1_2_12_25_1","unstructured":"Mills K. Kelly P. Tiraby JG. Lioux T. &Perouzel E.(2016).Patent of novel compound International Publication Number: WO 2016\/008946 A1; World Intellectual Property Organization."},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1002002718501"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784\u2010008\u20100218\u20104"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0241205"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.06180\u201011"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538\u20107836.2008.03219.x"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1055\/a\u20101827\u20107365"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00862\u201013"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.2RU0718\u2010290R"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.121.316108"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2006.06.027"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100037\u20101614344"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0006\u20102952(01)00760\u20102"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.18\u20100596"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109258(17)39154\u20108"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.3.1796"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2010.12.004"}],"container-title":["Molecular Oral Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/omi.12480","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T08:50:31Z","timestamp":1730710231000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/omi.12480"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,26]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/omi.12480"],"URL":"https:\/\/doi.org\/10.1111\/omi.12480","archive":["Portico"],"relation":{},"ISSN":["2041-1006","2041-1014"],"issn-type":[{"type":"print","value":"2041-1006"},{"type":"electronic","value":"2041-1014"}],"subject":[],"published":{"date-parts":[[2024,7,26]]},"assertion":[{"value":"2023-10-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}