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However, a full investigation has not been performed on the expression level of amyloid\u2010\u03b2 (A\u03b2) peptides in gingival crevicular fluid (GCF) and its effects on oral pathogens. This study aimed to analyze the expression level of A\u03b2 peptides in GCF of patients with periodontitis and the effects of A\u03b2 peptides against common oral pathogens. GCF samples were collected from patients with periodontitis (<jats:italic>n<\/jats:italic>\u00a0=\u00a015) and periodontally healthy people (<jats:italic>n<\/jats:italic>\u00a0=\u00a010). The antimicrobial effects of A\u03b2 peptides were evaluated on four common oral pathogenic strains using an MTT assay, crystal violet staining, fluorescence microscope, and transmission electron microscope. The protein levels of A\u03b240 and A\u03b242 were upregulated in the GCF of periodontitis group compared with the healthy group. Both A\u03b240 and A\u03b242 exhibited antimicrobial effects on <jats:italic>Porphyromonas gingivalis<\/jats:italic>, <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>, and <jats:italic>Lactobacillus acidophilus<\/jats:italic> in both planktonic and biofilm conditions. Further, only A\u03b240 showed an antimicrobial effect on the <jats:italic>Fusobacterium nucleatum<\/jats:italic>. The results of this study demonstrate that A\u03b2 peptides in GCF may be a relevant indicator of periodontitis status. Besides, the antimicrobial peptides derived from A\u03b2 peptides have great potential in periodontal therapy.<\/jats:p>","DOI":"10.1111\/omi.12488","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T06:33:37Z","timestamp":1734071617000},"page":"94-103","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Detection of Amyloid\u2010\u03b2 Peptides in Gingival Crevicular Fluid and Its Effect on Oral Pathogens"],"prefix":"10.1111","volume":"40","author":[{"given":"Yue","family":"Liao","sequence":"first","affiliation":[{"name":"Department of Periodontology Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology Shanghai Research Institute of Stomatology  Shanghai 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