{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:30:30Z","timestamp":1788348630313,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102019\u201006454"],"award-info":[{"award-number":["RGPIN\u20102019\u201006454"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Streptococcus mutans<\/jats:italic>\n                    , a key player in dental caries, faces multiple environmental challenges within the oral cavity, including oxidative stress, nutrient scarcity, and acidic pH. To survive and thrive,\n                    <jats:italic>S. mutans<\/jats:italic>\n                    has evolved intricate mechanisms, including the CSP\u2010ComDE quorum sensing system, which coordinates responses to environmental cues. The CSP\u2010ComDE system enables\n                    <jats:italic>S. mutans<\/jats:italic>\n                    to communicate with neighboring cells via its CSP pheromone. Under stress conditions, the CSP pheromone production increases, triggering a cascade of events. Notably, our research demonstrated that the CSP pheromone activates the expression of a Type II restriction\u2010modification (R\u2010M) system. Type II R\u2010M systems are well\u2010known tools in molecular biology and genetic engineering and consist of two distinct enzymes: a restriction enzyme and a methyltransferase. An increasing number of studies have revealed that bacterial adenine methylation (Dam methylation) has a broader role beyond mere DNA protection. In fact, the marks introduced into the DNA provide signals for a variety of physiological processes. Our results highlight a conserved chromosomal locus in\n                    <jats:italic>S. mutans<\/jats:italic>\n                    encoding the DpnII R\u2010M system. DpnII R\u2010M methylates DNA at 5\u2032\u2010GATC target sites within the\n                    <jats:italic>S. mutans<\/jats:italic>\n                    genome and cleaves unmarked DNA. Furthermore, our findings suggest that Dam methylation significantly impacts foreign DNA acquisition via natural transformation and modulates mutanobactin expression\u2014a secondary metabolite linked to oxidative stress tolerance. Collectively, our findings suggest that Dam methylation bridges epigenetics and bacterial fitness, potentially opening new avenues in bacterial epigenetics. As we explore this intricate biological process, we may uncover novel therapeutic strategies to combat bacterial infections.\n                  <\/jats:p>","DOI":"10.1111\/omi.12489","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T03:24:13Z","timestamp":1733196253000},"page":"82-93","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Decoding Adenine DNA Methylation Effects in\n                    <i>Streptococcus Mutans<\/i>\n                    : Insights Into Self\u2010DNA Protection and Autoaggregation"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0134-6495","authenticated-orcid":false,"given":"Haowei","family":"Zhao","sequence":"first","affiliation":[{"name":"Faculty of Dentistry University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Dufour","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Zhong","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siew\u2010Ging","family":"Gong","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul H.","family":"Roy","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Infectiologie, Centre de Recherche du CHU de Qu\u00e9bec Universit\u00e9 Laval  Qu\u00e9bec Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8044-7141","authenticated-orcid":false,"given":"C\u00e9line M.","family":"L\u00e9vesque","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry University of Toronto  Toronto Ontario 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