{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T06:08:01Z","timestamp":1771135681515,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n Tyrosine phosphorylation\/dephosphorylation post\u2010translational modification (PTM) of proteins in bacteria can control their function and location. PTM of transcriptional regulators and DNA\u2010binding proteins, as well as components of their signaling pathways, can impact gene expression. However, little is known regarding the global impact of tyrosine phosphatases on the bacterial transcriptome. In this study, we performed RNA\u2010Seq of\n Porphyromonas gingivalis<\/jats:italic>\n wild type (WT) along with strains \u0394\n ltp1<\/jats:italic>\n and \u0394\n php1<\/jats:italic>\n with mutations in the genes encoding the two major tyrosine phosphatases, Ltp1 and Php1, respectively. Moreover, these strains were tested in vitro and in vivo (mouse abscess) conditions. Both the \u0394\n ltp1<\/jats:italic>\n and the \u0394\n php1<\/jats:italic>\n mutants exhibited little transcriptional difference to the parental strain when cultured in vitro. In vivo, comparison of the\n \u0394php1<\/jats:italic>\n mutant to the WT showed a number of differentially regulated genes (DEGs) associated with transporter systems. In vivo DEGs in\n \u0394ltp1<\/jats:italic>\n included one of the efflux ABC transporter systems also regulated in the\n \u0394php1<\/jats:italic>\n mutant; however, the primary biological process populated by DEGs in\n \u0394ltp1<\/jats:italic>\n involved genome stability. Comparison of the WT strain between the in vitro and in vivo condition indicated that DNA metabolic processes, including recombination and transposition, were significantly upregulated in vivo. Hence, a major role of Ltp1 phosphatase activity at the transcriptional level may be control of adaptation to in vivo conditions. Additionally, both Ltp1 and Php1 have common functions in the control of the expression of genes encoding transporter systems.\n <\/jats:p>","DOI":"10.1111\/omi.70003","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T08:47:36Z","timestamp":1755679656000},"page":"258-264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptional Regulon Controlled by Tyrosine Phosphatases in\n Porphyromonas gingivalis<\/i>"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3694-2123","authenticated-orcid":false,"given":"Gatphan","family":"Atassi","sequence":"first","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases School of Dentistry University of Louisville Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kendall S.","family":"Stocke","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases School of Dentistry University of Louisville Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3147-5039","authenticated-orcid":false,"given":"Richard J.","family":"Lamont","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases School of Dentistry University of Louisville Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2540.2000.00751.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1717525115"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-003-0640-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14046"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2011.0424"},{"key":"e_1_2_9_7_1","first-page":"101","article-title":"Characterization of the Tripartite Drug Efflux Pumps of Porphyromonas gingivalis ATCC 33277","volume":"38","author":"Inoue T.","year":"2015","journal-title":"New 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