{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T04:26:20Z","timestamp":1776140780564,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The regulatory mechanisms of epithelial\u2013mesenchymal transition (EMT) involved in periodontitis pathogenesis are poorly understood. Consequently, this study aimed to investigate the association of the long noncoding (lnc) RNAs, NEAT1 and MALAT1, with EMT in periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Gingival tissue samples (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a057) were obtained from periodontitis patients indicated for surgical treatment and healthy control individuals. Full mouth periodontal charting was recorded for all patients together with collection of subgingival biofilm samples to determine bacterial load for key\u2010periodontal pathogens. Histopathological analysis was used to assess inflammatory cell infiltration, and RT\u2010qPCR analysis was performed to quantify the expression of the key EMT biomarkers of\n                      <jats:italic>E<\/jats:italic>\n                      \u2010cadherin, \u03b2\u2010catenin, Snail1 and vimentin, and the lncRNAs of Neat1 and Malat1.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The clinical parameters and percentage of inflammatory cell infiltration were significantly higher in the periodontitis group compared with healthy controls. In periodontitis, expressions of Malat1 and\n                      <jats:italic>E<\/jats:italic>\n                      \u2010cadherin were significantly downregulated, whereas Neat1, Snail1 and vimentin were significantly upregulated in comparison to controls. Receiver\u2010operating characteristic (ROC) analyses demonstrated moderate\u2010to\u2010good diagnostic accuracy of Neat1, Malat1, Snail1,\n                      <jats:italic>E<\/jats:italic>\n                      \u2010cadherin and vimentin (area under the curve [AUC]: 70.3%, 67.5%, 78.7%, 89.9% and 74.3%, respectively) to discriminate periodontal health from disease.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Probing pocket depth, bleeding scores, expression of Neat1, red complex bacteria (\n                      <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                      and\n                      <jats:italic>Treponema denticola<\/jats:italic>\n                      ) and downregulation of Malat1 and\n                      <jats:italic>E<\/jats:italic>\n                      \u2010cadherin were strongly associated with EMT. Data also highlighted an association between Neat1 and Malat1 with the induction of the EMT phenotype in periodontitis, and these lncRNAs may therefore provide novel diagnostic biomarkers.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/omi.70010","type":"journal-article","created":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T04:53:37Z","timestamp":1760158417000},"page":"38-47","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Long Noncoding RNA Malat1 and Neat1 Associated With Dysbiotic Microbiome and Epithelial\u2010Mesenchymal Transition in Periodontitis"],"prefix":"10.1111","volume":"41","author":[{"given":"Saif M.","family":"Al\u2010Mufti","sequence":"first","affiliation":[{"name":"College of Dentistry University of Baghdad  Baghdad Iraq"},{"name":"Iraqi Ministry of Health, Al\u2010Rissafa Directorate, SDR Specialized Dental Center  Baghdad Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0102-6715","authenticated-orcid":false,"given":"Ali A.","family":"Abdulkareem","sequence":"additional","affiliation":[{"name":"College of Dentistry University of Baghdad  Baghdad Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nibras H.","family":"Chasib","sequence":"additional","affiliation":[{"name":"College of Dentistry University of Baghdad  Baghdad Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mike","family":"Milward","sequence":"additional","affiliation":[{"name":"School of Dentistry University of Birmingham  Birmingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul R.","family":"Cooper","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry Sir John Walsh Research Institute University of Otago  Dunedin New 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